【摘要】 为了纯化海带褐藻糖胶及研究褐藻糖胶结构,利用DEAE-Sepharose Fast Flow离子交换柱分离海带褐藻糖胶粗品,得到LP-1、LP-2、LP-3和LP-4 4个组分,通过Sephadex G-150凝胶柱对硫酸根含量较高组分LP-2、LP-3和LP-4进行进一步分离纯化及分子量测定,最终LP-2获得两种均一多糖LP-21和LP-22,其分子量分别为123和105 k Da,LP-3和LP-4为均一多糖,分子量分别为74和32 k Da。通过高效液相色谱法分析得率较高的组分（LP-21、LP-22和LP-3）单糖组成,用红外色谱法分析其结构。高效液相色谱结果表明,分级纯化后的组分（LP-21、LP-22和LP-3）主要由L-岩藻糖、D-半乳糖组成,还有少量D-葡萄糖、D-甘露糖、D-氨基葡萄糖、L-鼠李糖、D-木糖和D-葡萄糖醛酸。红外光谱分析表明,LP-21和LP-22的硫酸根主要结合在岩藻糖C₄位,LP-3硫酸根主要结合在岩藻糖C₂或C₃位上。更多还原

【Abstract】 To purify crude fucoidan from Laminaria japonica and study the structure of fucoidan, crude polysaccharide in fucoidan from Laminaria japonica was separated by DEAE-Sepharose Fast Flow ion exchange column and four parts（LP-1, LP-2, LP-3 and LP-4） were obtained. LP-2, LP-3 and LP-4 that sulfate content higher were separated, purified and the molecular weight was determined by Sephadex G-150 gel column. LP-2 was separated into two homogeneous polysaccharides LP-21 and LP-22, the molecular weights were 123 and 105 k Da, LP-3 and LP-4 were homogeneous polysaccharides, and the molecular weights were 74 and 32 k Da. The monosaccharide composition of the high recovery rate component（LP-21, LP-22 and LP-3） was analyzed by high performance liquid chromatography（HPLC） and the structure of the product was analyzed by infrared spectroscopy（IR）. HPLC showed monosaccharides constituents for three polysaccharides were mainly composed of L-fucose, D-galactose, and few D-glucose, D-mannose, D-glucosamine, L-rhamnose, xylose, and D-glucuronic acid. The results of IR spectrum showed that their properties were the same as polysaccharides and sulfuric acid roots are incorporated into the C₄ of the diatom sugar for LP-21 and LP-22, sulfuric acid roots were incorporated into the C₂ or C₃ for LP-3.更多还原