选取牛、羊线粒体12S rRNA基因的特异性引物,以新鲜牛、羊乳样品提取模板DNA,建立了基于DNA结合染料(SYBR Green I)的实时荧光定量PCR方法,用于羊乳制品中牛乳成分的掺假鉴别检测。结果表明,该方法最低可检出新鲜羊奶中掺入2.5%的牛乳成分,并应用于11份市售羊乳制品样本中的牛、羊源性成分的鉴别。基于DNA结合染料的定量PCR检测无需电泳分离和设计标记DNA探针,具有快速、灵敏、低成本和高通量等优点,可以作为羊乳及其深加工制品中牛乳源性成分掺入检测的有效手段之一。
【英文摘要】
In this paper,a real-time PCR method based on SYBR Green I has been successfully established for the identification of bovines milk components in goat dairy products. Two pairs of specific primers targeted at mitochondrial 12 S rRNA genes were selected and DNA extracts from fresh bovine and goat milk samples were used as PCR templates. The results showed that the proposed method can detect the adulteration level of bovine milk as low as 2. 5% in fresh goat milk. The method was then applied in the identifica...
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