为拓展纤维素降解菌资源,将浓香和清香型白酒酒醅样品富集培养于羧甲基纤维素钠(sodium carboxymethyl cellulose,CMC-Na)液体培养基,利用变性梯度凝胶电泳技术(denaturing gradient gel electrophoresis,DGGE)和平板培养法研究富集培养物的细菌多样性,筛选高效纤维素降解菌。DGGE结果显示,原始酒醅样品和富集培养物的细菌多样性无显著差异(p<0.05),但群落结构相似性较低;富集培养物中的细菌分类于Acinetobacter、Bacillus、Klebsiella、Lactococcus、Paenibacillus。经刚果红水解圈、纤维素酶(carboxymethyl cellulase,CMCase)活力和滤纸条降解试验测定,获得3株纤维素降解力较强的细菌,分别为Paenibacillus sp.、Acinetobacter sp.和Gluconobacter sp.。由这3株细菌构建的复合菌系在液态发酵条件下对水稻秸秆和小麦秸秆均具有显著的降解作用,降解率分别为35.32%和28.89%。白酒酒醅蕴藏高效的纤维素降解菌,具有开发利用潜力。
【英文摘要】
Zaopei,a mixture of fermented grains used for Chinese Baijiu production,is a complex ecosystem that hosts diverse microbial communities that play important role in cereal fermentation. In this study,Cellulose-degrading bacteria(CDB)in Zaopei samples used for Strong-and Light-flavor Baijiu production were incubated in enrichment medium with sodium salt of caboxymethyl cellulose(CMC-Na)as the sole source of carbon. We performed denaturing gradient gel electrophoresis(DGGE)to explore the diversity of celluloly...
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
