This paper studied the effect of nattokinase production from Bacillus subtilis LSSE-22 in shake flask and bioreactor. Glucose and soy peptone were identified as the optimal carbon source and nitrogen source through batch fermentation in shake flask,thus were used in this study. Furthermore,the effect of feeding substrate,method and time on the nattokinase production was also studied at optimized initial glucose and soy peptone concentration.The results showed that feeding method produced 1 437. 34 IU/m L of...