【摘要】 目的建立固相萃取（solid phase extraction,SPE）结合超高效液相色谱-串联质谱法（ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS）测定瓶装饮用水中双酚A（bisphenol A,BPA）、双酚S（bisphenol S,BPS）的方法。方法利用BPA-D₄和BPS-¹³C₁₂混合同位素内标,经Waters Oasis系列的HLB固相萃取柱富集样品,以甲醇-水为流动相,经Waters BEH C₁₈色谱柱分离,采用UPLC-MS/MS在负离子电离（electron spray ionization,ESI-）模式和多反应监控（multiple reaction monitoring,MRM）模式下检测。结果用该方法测定时,BPA在1⁵0μg/L范围内线性良好,BPS在0.2¹0μg/L范围内线性良好,相关系数r均为0.9999,BPA的平均回收率为99.5%¹00.7%,相对标准偏差为0.8%⁴.6%;BPS的平均回收率为95.9%¹02.1%,相对标准偏差为1.3%².5%,BPA的最低检出浓度为20 ng/L,BPS的最低检出浓度为5 ng/L。结论此方法操作简单、灵敏度高、重现性好,适用于瓶装饮用水中双酚A、双酚S的检测。更多还原

【Abstract】 Objective To establish an ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） with solid phase extraction（SPE） method for the determination of bisphenol A（BPA） and bisphenol S（BPS） in bottled drinking water. Methods With BPA-D₄ and BPS-¹³C₁₂ isotope as mixed internal standards, the BPA and BPS were enriched by Waters Oasis HLB column. The samples were separated by Waters BEH C₁₈ chromatographic column with methanol-water as mobile phase, and then detected by UPLC-MS/MS under the multiple reactions monitoring（MRM） mode with the negative-ion mode and quantified by an internal standard method. Results Using the established method, BPA and BPS had good linearities in the ranges of 1-50 μg/L and 0.2-10 μg/L for BPA and BPS, respectively（r>0.999）. The average recoveries of BPA were 99.5%-100.7% and the relative standard deviations were 0.8%-4.6%. The average recoveries of BPS were 95.9%-102.1% and the relative standard deviations were 1.3%-2.5%. The least determination limits were 20 ng/L and 5 ng/L for BPA and BPS, respectively. Conclusion The established method is simple, sensitive and has good repeatability, which is suitable for the determination of BPA and BPS in bottled drinking water.更多还原