Cell-free protein synthesis system is one of modern protein expression systems based on cell extracts containing enzymes and protein factors, as well as substrates and energy substance in order to maintain the system operation. After adding an exogenous DNA template, the target protein could be ultimately synthesized in vitro. Cell-free protein synthesis system breaks the physiological limitations of cells, allows for flexible protein synthesis, and greatly improves the protein yield. It can be used not onl...
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