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基于菌落形态、显微特征、DNA条形码三种策略鉴定僵蚕表面真菌
Analysis of the Fungi Forms of the Bombyx Batryticatus Based on Three Tactics of Fungal Morphology, Microscopy and DNA Barcode
【摘要】 目的:检测北京市零售药店僵蚕饮片表面所含真菌的种类,为临床安全用药提供参考依据。方法:培养僵蚕表面真菌,进行纯化,获得单一菌株。结合真菌菌落形态、显微结构特征、DNA条形码三种方法鉴定僵蚕表面真菌。结果:从僵蚕饮片表面共获得真菌227株,共计10种。其中的8种真菌得到准确鉴定结果,2种真菌为未知菌株。结论:初步了解北京市零售药店僵蚕表面真菌情况,为临床安全用药提供参考依据。
【Abstract】 Objective: To detect the fungi kinds on the surface of Bombyx bassiana decoction in Beijing retail drugstoreand to provide reference for safe drug use in clinic. Method: The surface fungi of Bombyx bassiana were cultivated andpurified, and a single strain was obtained. Colony morphologies, microscopic characteristic and DNA barcode werecombined to identify fungi on the surface of Bombyx bassiana. Result: A total of 227 strains of fungi were obtained fromthe surface of Bombyx bassiana, which were 10 species, among which 8 species of fungi were obtained with accurateresults, 2 species were unknown strains. Conclusion: We have preliminary understanding of the surface fungi of bombyxbatryticatus in Beijing retail drug stores and provide a reference for the safety of the clinical medicine.
【Key words】 bombyx batryticatus; surface fungi; Aspergillus sp.; Penicillium sp.; Aspergillus niger;
- 【文献出处】 世界科学技术-中医药现代化 ,Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology , 编辑部邮箱 ,2018年07期
- 【分类号】R282.5
- 【下载频次】441