【摘要】 文章采用乙醇诱导再生柞蚕丝素蛋白(ASF)水溶液形成凝胶,并对该凝胶冷冻干燥制备出不溶于水的ASF多孔材料,利用傅里叶变换红外光谱(FTIR)和扫描电子显微镜(SEM)研究乙醇水溶液浓度、ASF初始浓度对ASF凝胶及其冻干后样品结构的影响,并采用激光扫描共聚焦显微镜(LSCM)对ASF多孔材料的生物相容性进行评价。FTIR结果表明,乙醇诱导ASF水溶液成胶伴随着大量β-折叠构象的形成。SEM结果显示,冻干后的ASF多孔材料孔径在14.2~30. 4μm。LSCM结果表明,该材料在生物医学领域具有潜在的应用价值。更多还原

【Abstract】 Antheraeapernyi silk fibroin(ASF) gels were prepared from regenerated ASF aqueous solutions induced by ethanol,and freeze-dried to form insoluble ASF porous materials. Fourier transform infrared spectroscopy(FTIR)and scanning electron microscope(SEM) were used to investigate the effects of ethanol concentration and original ASF concentration on the structure of ASF gel and its freeze-dried samples. Moreover,laser scanning confocal microscope(LSCM) was used to further evaluate the biocompatibility of the obtained ASF porous materials. FTIR results showed that the gelation process of regenerated ASF aqueous solution induced by ethanol was accompanied by the formation of substantial β-sheet conformation. SEM results indicated that the pore size of the obtained ASF freezedried samples ranged from 14. 2 to 30. 4 μm. LSCM results further showed that the ASF materials had great potential for biomedical application.更多还原