【摘要】 目的观察创伤应激后青春期大鼠饮酒行为的变化,并探讨下丘脑室旁核（hypothalamic paraventricular nucleus,PVN）中可卡因安非他明调节转录因子（cocaine-and-amphetamine-regulated transcript,CART）55-102的作用。方法模拟脏器探查术建立大鼠创伤应激模型。将青春期SD雄性大鼠随机分为创伤组和对照组（n=6）,采用双瓶饮酒模型测试连续14天的饮酒行为（包括饮酒量和酒精偏好）及饮酒结束后大鼠血清中乙醇浓度。采用免疫组化检测创伤组和对照组（n=6）大鼠PVN中CART55-102的免疫活性。采用立体定位法干预创伤组大鼠PVN中CART55-102的活性,分别注射生理盐水和CART55-102 （n=6）,测试后续饮酒行为,观察PVN中CART55-102在创伤应激诱导的饮酒行为中的作用。结果与对照组比较,创伤组大鼠的饮酒量(g·kg^-1·d^-1)、酒精偏好（%）以及测试结束后的血药浓度（mg/dL）明显增加（2.31±0.08 vs.1.76±0.19,16.79±0.53 vs.12.47±1.61,16.78±2.19 vs.8.85±1.69,P均<0.05）;且创伤组大鼠PVN中CART55-102的免疫活性大幅降低（0.76±0.07 vs.1.00±0.09,P<0.05）;与注射生理盐水组相比,CART55-102微注射组大鼠的饮酒量（1.05±0.12 vs.1.98±0.13,P<0.001）、酒精偏好（6.78±0.61 vs.10.13±0.73,P<0.01）及血药浓度（16.64±3.28 vs.29.83±3.82,P<0.05）明显降低。结论创伤应激可以增强青春期大鼠的饮酒行为,可能通过抑制PVN中CART55-102的免疫活性来发挥作用。更多还原

【Abstract】 Objective To observe the changes of drinking behavior after traumatic stress in adolescent rats,and explore the role of cocaine-and-amphetamine-regulated transcript（CART）55-102 of the hypothalamic paraventricular nucleus（PVN）. Methods Viscera exploration surgery was simulated to establish traumatic model in adolescence rats.Male SD rats were randomly divided into two groups（n=6）:control and trauma group,and measured the drinking behavior（including alcohol intake and preference）during 14 days by 2-bottle choice drinking paradigm and the level of ethanol in serum.The immune activity of CART55-102 in the PVN was detected through immunohistochemical staining.The activity of CART55-102 in the PVN was intervened by a stereotactic location method in traumatic rats injected by saline and CART55-102（n=6）.The drinking behavior was measured to explore the role of CART55-102 in drinking behavior induced by traumatic stress. Results Compared with control rats,alcohol intake,(g·kg^-1·d^-1)alcohol preference（%）and blood ethanol concentration（mg/dL）in traumatic rats increased statistically（2.31±0.08 vs.1.76±0.19,16.79±0.53 vs.12.47±1.61,16.78±2.19 vs.8.85±1.69,all P<0.05）,and the immune activity of CART55-102 in the PVN in traumatic rats decreased statistically（0.76±0.07 vs.1.00±0.09,P<0.05）.Further,CART55-102 injection into the PVN decreased alcohol intake（1.05±0.12 vs.1.98±0.13,P<0.001）,alcohol preference（6.78±0.61 vs.10.13±0.73,P<0.01）,and blood ethanol concentration（16.64±3.28 vs.29.83±3.82,P<0.05）compared with saline group. Conclusions Traumatic stress can stimulate drinking behavior in adolescent rats,possibly through inhibiting the immune activity of CART55-102 in the PVN.更多还原