【摘要】 目的研究角质细胞生长因子2(keratinocyte growth factor-2,KGF-2)对慢性阻塞性肺病(chronic obstructive pulmonary disease,COPD)模型小鼠肺部炎症及免疫功能的影响。方法取15只6～8周龄(体重20～25 g)的雄性BALB/C小鼠,首先用烟熏加气道滴注脂多糖(lipopolysaccharide,LPS)的方法构建COPD模型。KGF-2组于第1天和第14天给予KGF-2,共2次。采用肺组织HE染色及肺泡灌洗液(bronchoalveolar lavage fluid,BALF)蛋白浓度评价小鼠气道炎症及肺泡壁屏障的破坏。ELISA检测小鼠血浆及BALF中IL-6和肿瘤坏死因子(tumor necrosis factorα,TNF-α)的水平。流式细胞术检测小鼠脾脏中CD4+T细胞及CD8+T细胞的比例及细胞程序性死亡蛋白1(programmed cell death protein 1,PD-1)的表达。结果与COPD组小鼠比较,KGF-2组小鼠肺部炎症细胞浸润减少,肺泡结构相对完整,肺泡灌洗液及血浆中IL-6和TNF-α水平有降低的趋势。与COPD组比较,KGF-2组小鼠脾脏中CD4+T细胞及CD8+T细胞的比例升高(P<0.05),PD-1比例降低(P<0.05)。结论 KGF-2可能具有抑制COPD相关肺组织炎症及增强免疫功能的作用。更多还原

【Abstract】 Objective To study the effect of keratinocyte growth factor-2(KGF-2)on the lunginflammation in a mouse model of chronic obstructive pulmonary disease(COPD). Methods Fifteen BALB/C mice were randomly and equally divided into control,COPD and COPD with KGF-2 treatment groups.The COPD groups were exposed to cigarette smoke and lipopolysaccharide(LPS)instillation,and KGF-2 was instilled on day 1 and day 14 for KGF-2 group.The airway inflammationand alveolar barrier damage were evaluated by HE staining and bronchoalveolar lavage fluid(BALF)protein concentration respectively.The expression of IL-6 and TNF-a in plasma and BALF were detected by ELISA.The percentage of CD4+T cells,CD8+T cells and the expression of programmed cell death protein1(PD-1)in spleen of mice were detected by flow cytometry. Results Compared with the COPD mice,infiltration of inflammatory cells in the lungs and levels of IL-6 and tumor necrosis factorα(TNF-α)in alveolar lavage fluid and plasma were slightly decreased in KGF-2 group,with complete aveolar structure.CD4+T cells and CD8+T cells in the spleen increased(P<0.05),PD-1 ratio decreased(P<0.05)compared with the COPD mice. Conclusions KGF-2 has the effect of inhibiting COPD inflammation and enhancing immune function.更多还原