An HIV-1 cell-cell fusion system was developed to screen HIV-1 entry inhibitors that block cell-cell fusion. In this system, the p EGFP-Tat plasmid was constructed and cotransfected into effector cells(HEK-293 T) with HIV-1 envelope plasmid. TZM-bl cell, a genetically engineered cell line that expresses CD4, CXCR4, CCR5 as well as Tat-inducible β-galactosidase and luciferase reporter gene, was used as target cell. Thus, the co-culture of target cells and effector cells allows the cell fusion via Env and the...
Received:July 5,2017;Accepted:August 25,2017Supported by:National Natural Science Foundation of China(No.81202976),Scientific Research Project of Beijing EducationalCommittee(No.KM201510005029),National Science and Technology Major Project of the Ministry
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