【摘要】 探讨新疆黑蜂胶对结肠癌大鼠模型中T细胞的影响及其作用机制。雄性Wistar大鼠55只,随机分为对照组、模型组、蜂胶预防组、蜂胶治疗组和5-FU组。采用N-甲基-N’-硝基-N-亚硝基胍(MNNG)灌肠建立结肠癌大鼠模型。各治疗组给予相应药物治疗30d,其中蜂胶预防组于造模初便给予蜂胶预防性灌胃。每周观察大鼠体质量变化,HE染色观察结肠癌组织病理学变化、ELISA检测血清细胞因子TGF-β和IL-6水平、流式细胞术检测脾脏T细胞亚群数量、RT-PCR检测脾脏组织中转录因子Foxp3及ROR-γt mRNA的表达水平。结果显示,与模型组相比蜂胶治疗组及5-FU组大鼠体质量显著增高(P<0.05);模型组Treg数量及其转录因子Foxp3的表达较对照组明显增高(P<0.05),蜂胶预防组及5-FU组Treg数量及其转录因子Foxp3的表达较模型组明显降低(P<0.05);模型组Th17及其转录因子ROR-γt的表达较对照组明显增高(P<0.05),5-FU组Th17数量及其转录因子ROR-γt的表达较模型组显著降低(P<0.05);模型组大鼠Th17/Treg比值较对照组明显降低,而5-FU组大鼠Th17/Treg比值较模型组显著升高。这提示新疆黑蜂胶具有预防和治疗结肠癌的作用,调节相关T细胞的功能及细胞因子的表达可能是其作用机制之一。更多还原

【Abstract】 To investigate the effect of Xinjiang black propolis on T cells in colon cancer rat model and its mechanism,55 male Wistar rats were randomly divided into control group,model group,propolis prevention group,propolis treatment group and 5-FU group.Rat colon cancer model was established by N-methyl-N′-nitro-N-nitrosoguanidine(MNNG)enema.The treatment group was given corresponding drugs treatment for 30 days and the prevention group were given propolis by gavage just at the beginning of modeling.The body weight changes of rats were observed weekly,and the histopathological changes of colon cancer were observed by HE staining.The levels of serum cytokines TGF-βand IL-6 were detected by ELISA.The percentage of spleen T cells was detected by flow cytometry.The spleen tissue was examined by RT-PCR for the expression of transcription factors Foxp3 and ROR-γt mRNA.The results showed that compared with the model group,animal body weight of the propolis treatment group and the 5-FU group increased significantly after the treatment(P <0.05).The number of Treg cells and the expression of the Foxp3 in the model group were significantly higher than those in the control group(P < 0.05).The number of Treg cells and the expression of Foxp3 in propolis prevention group and 5-FU group were significantly lower than those of the model group(P<0.05).The number of Th17cells and the level of ROR-γt in the model group were significantly higher than those in the control group(P<0.05),but were significantly lower in the 5-FU group(P<0.05).The ratio of Th17/Treg in the model group was significantly lower than that of the control group(P<0.05),but was significantly higher in the 5-FU group than in the model group.Therefore,our study suggests Xinjiang black propolis may have a role in the prevention and treatment of colon cancer through regulating the function of related T cells and the expression of cytokines.更多还原