【摘要】 目的:评价国产多孔钽铌合金材料的骨结合性能。方法:选取成年新西兰大白兔36只,将多孔钽铌合金植体植入家兔股骨髁部,术后4、8、12周分批处死家兔并取材,通过X线片、硬组织切片、扫描电镜、X线能谱分析、推出试验评价其骨结合性能。采用SPSS19.0软件包对数据进行统计学分析。结果:X线检查未见植体松脱及明显骨吸收影像,随着愈合时间延长,材料周围骨密度增高;硬组织切片甲苯胺蓝染色观察显示,骨组织与多孔钽铌材料呈凹凸嵌合状,随着愈合时间延长,大量骨组织长入材料孔隙内部,4周组材料周围及内部多为类骨质,12周组材料周围及内部骨组织成熟度明显提高;扫描电镜与X线能谱分析显示,随着时间延长,骨组织与材料接触趋于致密,孔隙内骨组织钙磷元素百分含量逐渐增高,Ca/P比值8周组与12周组显著高于4周组(P<0.05),8周组与12周组之间无明显差异(P>0.05);推出试验示,4周时平均剪切强度为(8.26±0.75)MPa,12周时增加至(21.04±1.46)MPa,组间比较具有统计学差异(P<0.05)。结论:国产多孔钽铌合金材料在动物骨内具有优良的骨结合性能,是一种极具潜力的骨组织工程材料。更多还原

【Abstract】 PURPOSE: To evaluate the osseointegration of domestic porous tantalum-niobium(PTa-Nb) alloy.METHODS: A total of 36 adult New Zealand rabbits were selected and divided into 3 groups. Pta-Nb rods(3.5 mm×10 mm)were implanted into the femoral condyle of each rabbit.The rabbits were sacrificed successively at 4,8,12 weeks after operation. X-ray, hard tissue slices stained with toluidine blue, scanning electron microscope(SEM), X-ray energy disperse spectroscopy(XEDS) and push-out test were used to test the osseointegration of PTa-Nb. SPSS19.0 software package was used for statistical analysis. RESULTS: X-ray films showed no obvious inflammation,as well as implants loosing and bone resorption. Density of the bone around implants increased. Hard tissue slices displayed chimeric shape in the implant-bone interface. New bone contacted directly to the surface of PTa-Nb and got more and more closer with the increase of healing time. Osteoid formed in the inner pores at 4 weeks after operation,and more mature bone tissue grew into inner part of PTa-Nb at 12 weeks. SEM showed bone tissue was more denser and closer to the Pta-Nb,which was similar to the results of hard tissue slices. XEDS analysis showed the percent content of the calcium and phosphate of the tissue within PTa-Nb increased gradually in the three groups.The ratio of Ca/P at 8 and 12 weeks was significantly higher than at 4 weeks(P<0.05); Push-out test showed the shear-strength of PTa-Nb implants increased from(8.26±0.75) MPa at 4 weeks to(21.04±1.46) MPa at 12 weeks(P<0.05). CONCLUSIONS: The domestic PTa-Nb alloy has good osseointegration with both bone tissue ongrowth and ingrowth,which may be a high potential biomimetic bone material.更多还原