【摘要】 目的观察不同浓度的二甲双胍对人乳腺癌细胞增殖、凋亡、侵袭及PTEN表达的影响,并探讨其机制。方法将人乳腺癌细胞系SK-BR-3细胞分为4组,分别加入0、5、10、20 mmol/L的二甲双胍,记为0 mmol/L组、5mmol/L组、10 mmol/L组、20 mmol/L组。采用CCK-8法检测各组细胞增殖抑制率,采用流式细胞术Annexin VFITC/PI双染法检测各组细胞凋亡率,采用Transwell侵袭实验检测各组细胞侵袭能力,采用q RT-PCR法检测各组细胞PTEN mRNA,采用Western blotting法检测各组细胞PTEN蛋白。结果 0 mmol/L组、5 mmol/L组、10 mmol/L组、20 mmol/L组细胞增殖抑制率分别为0. 0%±0. 0%、23. 7%±2. 2%、43. 2%±2. 9%、63. 9%±5. 6%,细胞凋亡率分别为5. 1%±0. 2%、18. 2%±1. 9%、26. 3%±2. 0%、40. 1%±2. 6%,细胞穿膜细胞数分别为(170±23)个、(112±14)个、(74±9)个、(30±5)个,细胞PTEN mRNA的相对表达量分别为1. 01±0. 04、2. 89±0. 27、4. 12±0. 23、5. 45±0. 49,细胞PTEN蛋白的相对表达水平分别为0. 13±0. 05、0. 41±0. 06、0. 61±0. 10、0. 94±0. 11,上述各指标组间相比,P均<0. 05。结论 5、10、20 mmol/L的二甲双胍对人乳腺癌细胞增殖、凋亡、侵袭均可发挥调节作用,二甲双胍可能通过上调PTEN的表达调控乳腺癌细胞的增殖、凋亡和侵袭。更多还原

【Abstract】 Objective To observe the effects of different concentrations of metformin on proliferation,apoptosis,invasion,and PTEN expression of human breast cancer cells,and to explore its mechanism. Methods The human breast cancer cell line SK-BR-3 was divided into four groups: 0 mmol/L group,5 mmol/L group,10 mmol/L group,and 20 mmol/L group with treatment of corresponding concentrations of metformin. CCK-8 was used to detect the inhibition rate of cell proliferation,Annexin V-FITC/PI double staining method was used to detect the apoptotic rate of each group,Transwell invasion test was used to detect the invasive ability of each group,q RT-PCR was used to detect the PTEN gene of each group,and Western blotting was used to detect the PTEN protein of each group. Results The inhibition rates of cell proliferation in the 0 mmol/L group,5 mmol/L group,10 mmol/L group,and 20 mmol/L group were 0. 0% ± 0. 0%,23. 7% ± 2. 2%,43. 2% ± 2. 9%,and 63. 9% ± 5. 6%,and the apoptotic rates were 5. 1% ± 0. 2%,18. 2% ± 1. 9%,26. 3% ± 2. 0%,and 40. 1% ± 2. 6%,respectively. The number of cell penetrating the membrane in the 0 mmol/L group,5 mmol/L group,10 mmol/L group and 20 mmol/L group were 170 ± 23,112 ± 14,74 ± 9,and 30 ± 5,respectively. The relative expression levels of PTEN mRNA in the 0 mmol/L group,5 mmol/L group,10 mmol/L group,and 20 mmol/L group were 1. 01 ± 0. 04,2. 89 ± 0. 27,4. 12 ± 0. 23,and 5. 45 ± 0. 49,and the relative expression levels of PTEN protein were 0. 13 ± 0. 05,0. 41 ± 0. 06,0. 61 ± 0. 10,and 0. 94 ± 0. 11,respectively. Significant differences were found in the above indexes between groups( all P < 0. 05). Conclusion Metformin of 5,10,and 20 mmol/L can regulate the proliferation,apoptosis and invasion of human breast cancer cells. Metformin may regulate the proliferation,apoptosis and invasion of breast cancer cells by up-regulating the expression of PTEN.更多还原