【摘要】 目的观察异鼠李素(ISO)对炎症损伤小鼠巨噬细胞RAW264. 7增殖能力的影响,并探讨其机制。方法取对数生长期RAW264. 7细胞,将细胞分为5组,低、中、高剂量组分别加入已经配好含10 ng/m L脂多糖(LPS)及低、中、高剂量ISO(50、100、200μg/m L)的溶液,模型组加入含10μg/m L LPS的1640培养基(含10%FBS),对照组加入含10%溶剂对照的1640完全培养基。测算各组细胞增殖指数,检测各组一氧化氮(NO)、肿瘤坏死因子(TNF-α)。结果与对照组比较,模型组细胞12、24、36、48 h细胞增殖指数增加(P均<0. 05);与模型组比较,低剂量组24、36 h及中剂量组和高剂量组12、24、36、48 h细胞增殖指数减少(P均<0. 05);与低剂量组比较,中剂量组24 h及高剂量组24、48 h细胞增殖指数减少(P均<0. 05)。与对照组比较,模型组NO、TNF-α水平升高(P均<0. 05);与模型组比较,低剂量组NO水平及中剂量组和高剂量组NO、TNF-α水平降低(P均<0. 05);与低剂量组比较,中剂量组和高剂量组NO、TNF-α水平降低(P均<0. 05)。结论 ISO可抑制RAW 264. 7细胞增殖能力,且呈剂量依赖性,高剂量作用最大;机制可能与抑制NO、TNF-α有关。更多还原

【Abstract】 Objective To observe the effect of isorhamnetin( ISO) on the proliferation of macrophage RAW264. 7 in mice with inflammatory injury and to explore its mechanism. Methods RAW264. 7 cells in the logarithmic growth phase were divided into 5 groups: the low-,medium-and high-dose groups which were added with 10 ng/m L lipopolysaccharide( LPS) and low-,medium-and high-dose ISO solution( 50,100,and 200 μg/m L),the model group which was added with 10 μg/m L LPS in 1640 medium( containing 10% FBS),and the control group which was added with 1640 complete medium containing 10% solvent. The cell proliferation index of each group was measured,and nitric oxide( NO) and tumor necrosis factor-α( TNF-α) were detected in each group. Results Compared with the control group,the cell proliferation index of the model group increased at 12,24,36,48 h( P < 0. 05); compared with the model group,the cell proliferation index decreased in the low-dose group at 24 and 36 h,and the medium-dose group and the high-dose group at 12,24,36 and 48 h( P < 0. 05). Compared with the low-dose group,the cell proliferation index decreased in the mediumdose group at 24 h and the high-dose group at 24 and 48 h( P < 0. 05). Compared with the control group,the levels of NO and TNF-α in the model group increased( both P < 0. 05). Compared with the model group,the NO level in the low-dose group,and the NO and TNF-α levels in the medium-dose group and the high-dose group decreased( all P < 0. 05); compared with the low-dose group,the levels of NO and TNF-α decreased in the medium-dose group and the high-dose group( both P < 0. 05). Conclusion ISO can inhibit the proliferation of RAW 264. 7 cells in a dose-dependent manner by inhibiting the NO and TNF-α levels,and it has the greatest effect at the highest dose.更多还原