【摘要】 目的观察明目消朦（MMXM）预处理对H₂O₂诱导人视网膜色素上皮细胞（RPE细胞）凋亡和氧化应激损伤的影响。方法取对数生长的RPE细胞,将细胞分为4组,A组加入含MMXM的无血清培养基,MMXM的终浓度为0. 5 mg/m L,24 h后加入200μmol/m L的H₂O₂（以制备氧化应激损伤模型）; B组加入无血清的基础培养基,24 h后加入200μmol/m L的H₂O₂; C组加入含MMXM的无血清培养基,MMXM的终浓度为0. 5 mg/m L; D组加入无血清的基础培养基。比较各组细胞调亡率及ROS、MDA、SOD、GSH-PX。结果与B组比较,其余3组细胞凋亡率降低（P均<0. 05）;与C、D组比较,A、B组细胞凋亡率升高（P均<0. 05）。与D组比较,A、B组ROS（+）、MDA升高,ROS（-）、SOD、GSH-PX降低（P均<0. 05）;与B组比较,其余3组ROS（-）、SOD、GSH-PX升高,ROS（+）、MDA降低（P均<0. 05）。结论 MMXM预处理能有效减少H₂O₂诱导的RPE细胞凋亡,并可预防细胞氧化损伤。更多还原

【Abstract】 Objective To observe the effects of pretreatment of Mingmu Xiaomeng（ MMXM） on H₂O₂-induced apoptosis and oxidative stress injury in human retinal pigment epithelial cells（ RPE cells）. Methods RPE cells in the logarithmic growth were divided into 4 groups: groups A,B,C,and D. Cells in the group A were added with serum-free medium containing 0. 5 mg/m L MMXM,and 200 μmol/m L H₂O₂ was added after 24 h（ to prepare an oxidative stress damage model）; serum-free basal medium was added to the cells in the group B,and 200 μmol/m L H₂O₂ was added after 24 h;cells in the group C were added with serum-free medium containing MMXM with the final concentration of 0. 5 mg/m L; serum-free basal medium was added to the cells in the group D. The apoptosis rate and reactive oxygen species（ ROS）,malondialdehyde（ MDA）,superoxide dismutase（ SOD） and glutathione peroxidase（ GSH-PX） were analyzed. Results Group B had a higher apoptosis rate than the other three groups（ P < 0. 05）; compared with group C and D,the apoptosis rate increased in the group A and group B（ P < 0. 05）; compared with group D,ROS（ +） and MDA increased,and ROS（-）,SOD,and GSH-PX decreased in the group A and group B（ all P < 0. 05）; compared with group B,ROS（-）,SOD,and GSH-PX increased and ROS（ +） and MDA decreased in the other three groups（ all P < 0. 05）. Conclusion MMXM pretreatment can effectively reduce H₂O₂-induced apoptosis and prevent oxidative damage in RPE cells.更多还原