【摘要】 目的:研究分化相关基因NDRG2在胃癌细胞中的表达及与细胞分化的关系。方法:采用RT-PCR及Western blot检测胃永生化粘膜上皮细胞GES1及6株胃癌细胞株中NDRG2的m RNA及蛋白的表达情况,进一步采用分化诱导剂TPA处理胃癌细胞株,分析NDRG2的表达水平及恶性表型是否可逆转。结果:与胃永生化粘膜上皮细胞GES1相比,NDRG2在6株胃癌细胞中有不同程度的表达下调,未分化胃癌细胞株HGC27中表达最低(P <0. 05)。不同浓度的分化诱导剂TPA(0、10、50、100nmol/L)处理胃癌细胞株HGC27后,随TPA浓度升高,NDRG2表达上调,100nmol/L组表达最高(P <0. 05),Transwell实验显示TPA干预组胃癌细胞的侵袭力明显减弱。结论:分化诱导剂干预可上调胃癌细胞NDRG2表达水平,逆转细胞恶性表型,且与细胞分化成正相关,故NDRG2可能是胃癌中一种候选的肿瘤抑制基因。更多还原

【Abstract】 Objective: To investigate the expression of NDRG2 in gastric cancer cell lines and its relationship with cell differentiation. Methods: Protein and m RNA level of NDRG2 were detected in GES1 and 6 gastric cancer cell lines by RTPCR and western blot,then TPA was utilized to treat gastric cancer cell line( HGC27) to detect NDRG2’s expression and invasion. Results: The expression of NDRG2 was down-regulated in gastric cancer cell lines compared to GES1,and it was the lowest in HGC27. After treatment with TPA in HGC27,the expression of NDRG2 significantly increased,and its invasion ability obviously weakened. Conclusion: It suggests that NDRG2 may be a tumor suppressor gene in gastric cancer,and it is closely related to cell differentiation.更多还原