【摘要】 本文就甘薯收获后,自然储藏期间淀粉酶种类变化和部分性质进行研究.从收获开始,每间隔2周取样1次,按3mL/g加入缓冲液,提取可溶性总蛋白,经8%连续聚丙烯酰胺凝胶电泳(PAGE)及淀粉酶活性染色,结果显示:在贮藏期间,有新的淀粉酶活性带出现,在性质上与原有淀粉酶活性带存在差异.主要表现在,对β-巯基乙醇和EDTA均非常敏感,而原有淀粉酶活性带不敏感;在热稳定性方面新出现的淀粉酶也更为敏感.说明甘薯块根在贮藏期间,新陈代谢旺盛,有新的淀粉酶表达,是需要二硫键的金属酶.更多还原

【Abstract】 The part characteristics and the difference of amylase were investigated in tuberous root of sweet potato,during storage period.The total soluble protein were extracted with 3 mL/g buffer,it harvests between 2 weeks in the storage time.Difference amylases were found in isozyme composition in tuberous roots of sweet potato during storage period,by electrophoresis on 8% native polyacrylamide gel and active stain by using soluble starch as substrate.New amylase responds differently to inhibitors,it is sensitive to EDTA or thiol-group reagent,and it is more sensitive to temperature thanβ-amylase.New amylase can be metalloenzyme with disulfide bond.更多还原