【摘要】 目的:研究真武汤及温阳组、利水组对肾小管上皮细胞氧化损伤的干预作用,为中医治则治法的科学性提供实验依据。方法:不同浓度H2O2作用于肾小管上皮细胞HK-2,诱导HK-2细胞氧化损伤模型,采用真武汤组含药血清、温阳组含药血清、以及利水组含药血清进行干预,分别检测各中药组对损伤细胞的增殖作用,对缺氧损伤相关基因HO-1、HIF-1α、生存素(s urvivin)表达的影响,以及对肾纤维化和肾小管上皮转分化相关基因转化生长因子β1(TGF-β1)、α-平滑肌肌动蛋白(SMA)、钙黏蛋白(E-c a dherin)基因表达的影响。结果:H2O2作用不同时间可造成HK-2细胞损伤明显。与模型组比较,真武汤组和利水组具有明显修复HK-2细胞生长的作用(P<0.05),温阳组作用不显著;真武汤组、温阳组及利水组均可上调HO-1、HIF-1α的表达,抑制s urvivin的表达(P<0.05),但三者之间差异不明显。真武汤组、温阳组及利水组可降低SMA及E-c a dherin的表达,温阳组可下调TGF-β1的表达,差异均有统计学意义(P<0.05)。结论:真武汤对体外HK-2细胞氧化损伤模型具有明显的保护作用,利水组对损伤模型的修复作用比较明显,温阳组抗肾纤维化及肾小管上皮转分化作用比较明显。本研究为中医治则治法的科学性提供了实验依据。更多还原

【Abstract】 Objective: To study the intervention effects of Zhenwu tang and its decomposed recipes on the oxidative damage of renal tubular epithelial cells and to provide experimental evidence for the scientific character of principles of the Chinese medicine treatment. Methods:Used different concentrations of H2 O2 to act on renal tubular epithelial cells HK-2 to form a model of the oxidative damage in renal tubular epithelial cells. Intervened by the group of Zhenwu tang in serum containing drug, the group for warming yang in serum containing drug and the group for promoting diuresis in serum containing drug, and detected the proliferation effect in injured cells in each Chinese medicine group as well as the effects on the expressions of hypoxia-injured genes(HO-1, HIF-1α and survivin)respectively. Detected the effects on renal fibrosis and the expressions of relative genes of renal tubular epithelial transdifferentiation,such as TGF-β1,SMA and E-cadherin. Results:H2 O2 can cause significant damages to HK-2 cells at different times. Compared with the model group,the group of Zhenwu tang and the group for promoting diuresis had a significant effect in repairing HK-2 cells(P <0.05),while the group for warming yang had no obvious effect. The group of Zhenwu tang,the group for warming yang and the group for promoting diuresis can elevated the expressions of HO-1, HIF-1α genes and had a significant effect in inhibiting survivin genes.when compared with the model group(P < 0.05), but the differences among the three groups were not evident. The group of Zhenwu tang,the group of warming yang and the group for promoting diuresis can reduce the expressions of SMA and E-cadherin genes;and the group of warming yang can reduce the expressions of TGF-β1,differences being significant(P < 0.05). Conclusion:Zhenwu tang has an obvious effect in protecting the model of oxidative damage renal tubular epithelial cells in vitro. the group for promoting diuresishas a more significant effect in repairing the injured model and the group for warming yang has an evident effect in ameliorating renal fibrosis and renal tubular epithelial transdifferentiation. This study provides an experimental basis for the scientific character of principles of Chinese medicine treatment.更多还原