【摘要】 目的按照自贡市疾病预防控制中心编制的艾伯特埃希菌的检测程序,对四川荥经县采集的鸡肠样品中艾伯特埃希菌的携带情况进行检测,了解该地区家禽中艾伯特埃希菌的携带情况及菌株特征。方法采集荥经县农贸市场、棒棒鸡店铺、活鸡宰杀点的新鲜鸡肠样品163份,按照艾伯特埃希菌的检测程序对样品进行检测,鸡肠内容物经EC肉汤增菌后,PCR检测eae基因,eae阳性样品的增菌液接种于麦康凯琼脂,37℃,24 h培养后挑取不发酵乳糖、eae阳性的疑似菌株,使用多重PCR检测及多位点序列分析(MLST)对疑似菌株进行鉴定。结果自贡市疾病预防控制中心编制的艾伯特埃希菌的检测程序可行有效,在荥经县共采集了新鲜的鸡肠样163份品,从3份样品中分离到3株疑似艾伯特埃希菌菌株,经多重PCR检测及多位点序列分析(MLST)鉴定,3株疑似菌株均为艾伯特埃希菌。本次采集的样品艾伯特埃希菌的检出率为1.84%(3/163)。结论四川荥经县地区鸡肠中存在艾伯特埃希菌,分离到的菌株丰富了艾伯特埃希菌生物学特征、流行病特征研究的菌株数据库。更多还原

【Abstract】 Objective In order to investigate the prevalence and characteristics of Escherichia albertii in Yingjing, we detected the Escherichia albertii carrying status of the chicken intestine samples collected from Yingjing Sichuan Province according to the detection procedure of Escherichia albertii edited by Zigong Center of Disease Control and Prevention(Zigong CDC).Methods A total of 163 samples of chicken intestines which were collected from open market of agricultural products,Bangbang chicken shop, slaughtering location of chicken and so on, were detected according to the detection procedure. The chicken intestines were enriched with EC Medium(Escherichia coli Medium), the positive PCR detection of eae gene for chicken intestines were inoculated in MacConkey Sorbitol Agar(MSA) for 24 hours on 37 °C. The eae-positive lactose non-fermenting isolates further classified as Escherichia albertii by multiplex PCR and multilocus sequence typing(MLST).Results The detection procedure edited by Zigong CDC was feasible and effective. One hundred and sixty-three chicken intestines samples were collected in this study, three suspected strains were detected from three samples, and which were classified as Escherichia albertii by multiplex PCR and MLST. The detection rate was 1.84%(3/163).Conclusions This study indicate that Escherichia albertii is existed in Yingjing, and the strains isolated in this study enrich the strain database of biological characteristics and epidemiological studies of Escherichia albertii.更多还原