【摘要】 目的检测Toll样受体(TLR)2在耐多药肺结核患者外周血中性粒细胞(PMN)的水平,探讨其在结核分枝杆菌耐药机制中的意义。方法在药物干预前后,应用酶联免疫吸附试验(ELISA)技术,分别检测26例耐多药肺结核患者、30例非耐药肺结核患者和30例正常对照者外周血PMN培养上清液中TLR2的水平。结果干预前耐药组肺结核(PTB)患者外周血PMN培养上清液中TLR2的平均浓度为(11.789 6±3.346 0)ng/mL,低于非耐药组PTB患者外周血PMN培养上清液中TLR2的平均浓度(17.878 0±1.326 4)ng/mL(P=0.000)。同时,耐药组TLR2水平也低于正常对照组(14.719 7±2.635 4)ng/mL(P=0.001)。干预前后,耐药组TLR2水平差异无统计学意义,而非耐药组患者外周血PMN上TLR2的水平与干预前相比有降低(P=0.000)。结论 TLR2可能在耐多药结核分枝杆菌对宿主产生免疫逃避上发挥了关键作用,对于其具体作用机制,仍需进一步研究。更多还原

【Abstract】 Objective To detect the levels of TLR2 on peripheral blood polymorphonuclear cell(PMN)in patients with multidrug-resistant pulmonary tuberculosis(MDR-PTB)and explore their significance in the drug resistance mechanism of Mycobacterium tuberculosis.Methods Totally 56 patients with MTB were divided into two groups:MDR-MTB group(n=26)and non-MDR-MTB group(n=30).Meanwhile,30 healthy volunteers were assigned as control group.Followed by any treatments,ELISA assays were employed to detect TLR2 levels in the supernatant of peripheral blood PMN.After systemic treatments,TLR2 levels in MTB patients of two groups were detected again.Results Before treatment,the TLR2 average level of PMN culture supernatant in MDR-MTB group was(11.789 6±3.346 0)ng/mL,which was significantly lower than non-MDR-MTB group(17.878 0±1.326 4)ng/mL and control group(14.719 7±2.635 4)ng/mL(,P=0.000,P=0.001,respectively).There was no statistic difference of TLR2 in MDR-MTB group pre-and post-the treatment.However,the TLR2 levels decreased significantly in non-MDR-MTB after treatment,compered with pre-treatment(P=0.000).Conclusion These finding indicated that TLR2 may play the critical roles in immune evasion of MDR-MTB.However,more studies should be carried out to further evaluation.更多还原