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采用植物组织培养消毒技术分离河口地区香蕉内生真菌的初步研究

Preliminary Research on the Isolation of Endophytic Fungi by Plant Tissue Culture and Disinfection Techniques of Banana Plants in Hekou

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【作者】 张建春柳青李春李芹

【Author】 ZHANG Jianchun;LIU Qing;LI Chun;LI Qin;Honghe Institue of Tropical Agricultural Sciences of Yunnan;Department of Resources and Environmental Sciences, Baoshan College;

【机构】 云南省红河热带农业科学研究所保山学院资源与环境学院

【摘要】 对河口地区主栽的不同品种香蕉分3条线路进行样本采集,对采集到的香蕉组织进行内生真菌分离、鉴定,共分离到9属494株内生真菌。鉴定结果表明,刺盘孢属(Colletotrichun sp.)、曲霉属(Aspergillus sp.)、青霉属(Penicillin sp.)为优势菌群。这3种优势菌群在东部路线、中部路线、西部路线的分离频率分别为78.37%、79.55%、76.54%;在巴西蕉,威廉斯,红研一号、红研二号中的分离频率分别为84.19%、77.32%、79.60%、71.48%。表明香蕉植株组织内内生真菌具有多样性,为进一步研究提供基础数据。

【Abstract】 The sample collection of different varieties of Banana was carried out from 3 lines, the endophytic fungi from the tissue specimens was isolated and identified. About 494 strains of endophytic fungi were obtained, they could be classified into 9 genera. Colletotrichun, Aspergillus and Penicillin were dominant fungi. The isolation frequency of these three dominat fungi in the eastern direction, central direction and western direction were 78.73%, 79.55% and 76.54%, respectively. The isolation frequency were 84.19%, 77.32%, 79.60% and 71.48% in varieties of Ba Xijiao, Weilians, Hongyan 1 and Hongyan 2.It was concluded that the endophytic fungi species of Banana plants in Hekou area had great diversity,which could provide references for the further research.

【关键词】 河口香蕉内生真菌物种多样性
【Key words】 Hekoubanana plantsendophyticfungispecies diversity
【基金】 云南省应用基础研究计划项目“河口地区香蕉内生真菌的物种多样性及寄主专一性研究”(No.2015DF086)
  • 【文献出处】 热带农业科学 ,Chinese Journal of Tropical Agriculture , 编辑部邮箱 ,2018年01期
  • 【分类号】S668.1
  • 【下载频次】161
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