【摘要】 目的评估miR-370在原发性中枢神经系统淋巴瘤(PCNSL)的表达和预后意义。方法通过即时聚合酶链锁反应(qRT—PCR)测定62例PCNSL的miR-370的表达水平,并以12例患者的颈部淋巴结组织进行miR-370测定作为对照。并对62例PCNSL患者的临床特征、手术以及治疗方案、生存时间等进行收集和随访。结果 miR-370在PCNSL组织中相对表达比较水平明显低于淋巴结组织,差异有统计学意义(t=5.11,P<0.05)。单一脑叶患者的miR-370表达明显多于多个脑叶患者,差异有统计学意义(t=7.13,P<0.05)。不同性别、年龄、不同的手术方式、治疗方案组间miR-370表达差异无统计学意义(t分别=2.77、1.81、3.05、2.43,P均>0.05)。年龄、肿瘤位置、手术方案、miR-370的表达水平是影响PCNSL预后的影响因素(HR分别=0.11、1.95、0.37、0.01,P均<0.05)。结论 miR-370在PCNSL细胞中异常低表达,是一个重要的预后预测因子。此外,年龄、肿瘤位置和手术方案也是影响PCNSL患者预后的重要因素。更多还原

【Abstract】 Objective To evaluate the expression and prognostic significance of miR-370 in primary central nervous system lymphoma(PCNSL). Methods Immediate polymerase chain reaction(qRT-PCR)was used to detect the expressions of miR-370 in 62 cases of PCNSL,and miR-370 was detected in 12 cases of cervical lymph node tissues as control. The clinical features,surgical approach,treatment and median survival time of the patients were followed-up.Results The relative expression level of miR-370 in PCNSL tissues was significantly lower than that in lymph node tissues(t=5.11,P<0.05). The expression of miR-370 in patients with single lobe was significantly higher than that in patients with multiple lobes(t=7.13,P<0.05). There was no significant difference in the expression of miR-370 between the groups of different sex,age,surgery method and treatment plan(t=2.77,1.81,3.05,2.43,P>0.05). Age,tumor location,surgical procedure,and the expression of miR-370 were the influencing factors for the prognosis of PCNSL(HR=0.11,1.95,0.37,0.01,P<0.05). Conclusion The miR-370 is an important prognostic factor with the low expression in PCNSL. In addition,age,tumor location and surgery method are also important factors that were related to the prognosis of PCNSL.更多还原