【摘要】 目的研究酒精对人牙周膜细胞RANKL和OPG表达的影响。方法酶消化法结合组织块法分离培养人牙周膜细胞,取4-6代细胞,在0.05 mol/L、0.10 mol/L、0.20 mol/L等不同浓度酒精环境下培养6、12、24、48h,RT-PCR检测RANKL、OPG m RNA的表达。采用SPSS 18.0软件包,对数据进行单因素方差分析。结果 0.05 mol/L、0.10 mol/L、0.20 mol/L浓度实验组RANKL m RNA表达量均高于对照组,具有统计学差异(P<0.05),并且随着酒精浓度的升高,RANKL m RNA表达逐渐升高。0.05 mol/L、0.10 mol/L、0.20mol/L浓度实验组OPG m RNA表达量均低于对照组,具有统计学差异(P<0.05),并且随着酒精浓度的升高,OPG m RNA的表达逐渐下降。结论不同浓度的酒精可以影响人牙周膜细胞中RANKL和OPG m RNA表达,在牙周炎骨吸收中起促进作用。更多还原

【Abstract】 Objective To explore effect of alcohol with different concentration on m RNA expression of RANKL and OPG in h PDLCs.Methods The primary h PDLCs were cultivated with enzyme digestion assay and tissue cultivation.The 4-6 generation of h PDLCs were respectively cultured 6,12,24,48 h in 0.05 mol/L、0.10 mol/L、0.20 mol/L concentration of alcohol.The m RNA expression of RANKL and OPG was detected with RT-PCR.The experimental data was analyzed by one way ANOVA using SPSS 18.0.Result The m RNA expression of RANKL in 0.05 mol/L、0.10 mol/L、0.20 mol/L Concentration alcohol were higher than control group.They increased gradually with the increase of alcohol concentration.The different was significant(P<0.05). The m RNA expression of OPG in 0.05 mol/L、0.10 mol/L、0.20 mol/L concentration alcohol were lower than control group.They decreased gradually with the increase of alcohol concentration.The difference was significant(P<0.05).Conclusion The alcohol with different concentration can regulate the m RNA expression of OPG and and OPG in h PDLCs and will promote bone resorption in periodontitis.更多还原