【摘要】 目的探讨氟达拉滨(FDR)及马磷酰胺(MFA)诱导CD19嵌合抗原受体T细胞(CD19-CAR-T)凋亡的信号通路,为研究化疗药物对CD19-CAR-T细胞抑制作用的机制奠定理论基础。方法 FDR及MFA与CD19-CART细胞相互作用后采用Annexin V/PI双染法检测CD19-CAR-T细胞膜的变化。结果经过FDR及MFA处理过的CD19-CAR-T细胞相对于未经处理过的细胞,凋亡率明显增加,差异有统计学意义(P<0.05)。结论通过细胞膜的变化可以判断FDR及MFA对CD19-CAR-T细胞具有诱导早期凋亡的作用。更多还原

【Abstract】 Objective To investigate the fludarabine and mafosfamide how to induced the apoptosis of CD19-CAR-T cells and lay the theoretical foundation for the further research. Methods Fludarabine and mafosfamide incubated with CD19-CAR-T cells sometimes and annexin V/PI double staining method was used to detect the CD19-CAR-T cells of early apoptosis to determine the CD19-CAR-T cells of apoptotic pathways induced by drugs. Results After fludarabine and mafosfamide treated CD19-CAR-T cells compared with unnot treated cells,the apoptosis rate increased significantly( P < 0. 05). Conclusion The changes of cell membrane can judge the fludarabine and mafosfamide how to induce the apoptosis of CD19-CAR-T cells at the early stage.更多还原