【摘要】 [目的]考察提取溶剂种类及用量、净化剂和色谱柱4大因素对联苯菊酯在茶叶和土壤中的提取和分析的影响。建立优化的气相色谱检测茶叶和土壤中联苯菊酯残留的分析方法,提高测定效率。[方法]在单因素条件下设计了正交试验L₉（3⁴）,采用方差分析考察各因素的影响,得到最佳条件。[结果]在优化的条件下,联苯菊酯在0.05⁴0.00 mg/L范围内具有较好的线性关系,其相关系数均大于0.999,在土壤和茶叶中的检出限分别为1.22、2.04μg/kg,定量限分别为4.05、6.81μg/kg。在0.05、0.40、4.00、8.00 mg/kg 4个添加水平下,联苯菊酯在茶叶和土壤中的回收率分别为87.92%⁹8.53%和87.53%¹00.46%,日内相对标准偏差分别为2.09%⁴.33%和3.52%⁵.46%,日间分别为3.33%⁵.78%和1.27%⁴.53%。[结论]该检测方法快速简便、定量准确、精密度良好,可以满足联苯菊酯在茶叶和土壤实际样品中残留检测的要求。更多还原

【Abstract】 [Aims] To optimize the pretreatment method for detecting bifenthrin in tea and soil, the effects of the kinds and dose of extraction solvents, absorbents and columns on the extraction and detection were investigated to gain best extraction and analysis efficiency. An optimized gas chromatography method was developed to determine the residues of bifenthrin in tea and soil. [Methods] Based on the single factor experiments, the orthogonal test L₉（3⁴） was designed and analysis of variance was employed to analyze the role of each factor and perform method validation for determination of bifenthrin in the two matrices. Thus, get the best condition. [Results] Employing the optimized method, good linearity was obtained（correlation coefficient > 0.999） in the range of 0.05-40.00 mg/L. The limits of detection for bifenthrin in soil and tea were 1.22 and 2.04 μg/kg, respectively. The limits of quantification for bifenthrin in soil and tea were 4.05 and 6.81 μg/kg, respectively. With the matrix-matched calibration, the average recoveries of bifenthrin in tea and soil at four spiked levels were 87.92-98.53 and 87.53-100.46%, respectively. The relative standard deviations with intra-day were 2.09-4.33 and 3.52-5.46%, respectively. The relative standard deviations of bifenthrin with inter-day were 3.33-5.78 and 1.27-4.53%, respectively. [Conclusions] The method is quick, easy, effective,sensitive and accurate, which can be applied to the residual analysis of bifenthrin in actual tea and soil samples.更多还原