【摘要】 长链非编码RNA (long noncoding RNA, LncRNA)参与哺乳动物多种生物学过程,目前研究其在毛囊形成过程中的作用机制却相对较少,因此研究LncRNA对毛囊发育的影响成为了解毛囊形成生物学过程的新方向。为验证前期获得的苏博美利奴羊(Ovis aries)毛囊发育不同时期皮肤组织的RNA-Seq结果以及与预测靶基因表达量差异,本研究利用qRT-PCR技术检测了在苏博美利奴羊毛囊发育6个关键时期两种在皮肤组织中差异表达且靶基因与毛囊发育相关的LncRNA 00202353和LncRNA 00316173及其靶基因的表达情况。结果表明,LncRNA 00202353及其靶基因分泌型卷曲相关蛋白2 (secreted frizzledrelated protein 2, SFRP2)的表达量在胚胎期85 d极显著高于胚胎期65 d、胚胎期135 d、出生后7 d和出生后30 d的表达量(P<0.01);LncRNA 00316173及其靶基因胰岛素样生长因子2 (insulin-like growth factor2, IGF2)的表达量在胚胎期65 d时极显著高于胚胎期135 d、出生后7 d和出生后30 d的表达量(P<0.01);将苏博美利奴羊毛囊发育时期分为发育期和成熟期进行分析,发现LncRNA 00202353及其靶基因SFRP2与LncRNA 00316173及其靶基因IGF2的表达量在毛囊发育期极显著高于毛囊成熟期(P<0.01),研究表明LncRNA 00202353与LncRNA 00316173及其靶基因参与毛囊的发育过程。本研究结果对后期深入研究LncRNA对毛囊发育的研究具有重要的参考价值,也为探究超细毛羊毛囊周期性变化的生物学过程提供研究线索。更多还原

【Abstract】 Long noncoding RNA(LncRNA) is involved in a variety of biological processes in mammals, andthe current research on its role in hair follicle formation process is relatively small, therefore, studying the effect of LncRNA on hair follicle development has become a new direction to understand the biological process of hair follicle formation. In order to verify the RNA-Seq results of skin tissue in the non-period of the development of the Subo Merino sheep(Ovis aries) and the difference in the expression of predicted target genes, the expression of two LncRNA 00202353 and LncRNA 00316173 and their target genes in skin tissue,which were differentially expressed in skin tissue during the development of Subo Merino sheep and their target genes associated with hair follicle development were detected by qRT-PCR. The results showed that the expression level of LncRNA 00202353 and its target gene secreted frizzled-related protein 2(SFRP2) at 85 day of embryonic stage was extremely significantly higher than 65 and 135 d of embryonic stage, 7 and 30 d after birth(P<0.01). The expression level of LncRNA 00316173 and its target gene insulin-like growth factor2(IGF2) at 65 d of embryonic stage was extremely significantly higher than 135 d of embryonic stage, 7 and30 d after birth(P<0.01). Analysis of the hair follicle developmental stage of the Subo Merino sheep was divided into developmental and maturity stages, the expression levels of LncRNA 00202353 and its target gene SFRP2, LncRNA 00316173 and its target gene IGF2 were extremely significantly higher in the hair follicle development than in the hair follicle maturation(P<0.01). It indicatd that LncRNA 00202353 and LncRNA 00316173 and their target genes were involved in the development of hair follicles. These resullts provide important reference value for further studies on the development of hair follicles in LncRNAs, and supply research clues for exploring the biological processes of the periodic changes of ultrafine woolen sacs.更多还原