【摘要】 为探讨蜂王浆主蛋白(MRJPs)对四氯化碳(CCl4)引起小鼠急性肝损伤的保护作用,建立CCl4诱导的小鼠急性肝损伤模型,将模型小鼠随机分组,每组8只,空白对照组、模型组、药物对照组(联苯双酯)以及MRJPs低、中、高剂量组),检测各组小鼠血清中谷丙转氨酶(AST)、谷草转氨酶(ALT)等指标的变化,测定肝组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性的变化,利用光镜观察肝组织病理形态学变化并测定肝脏中肿瘤坏死因子-α的基因表达量。结果表明:预先灌胃给予MRJPs的小鼠,可以显著抑制CCl4诱导的血清ALT和AST活性水平(P<0.01);MRJPs可以显著抑制MDA的生成(P<0.01),从而抑制肝脏脂质过氧化;MRJPs可以显著抑制CCl4诱导的小鼠肝脏SOD活性的降低(P<0.01),从而增强小鼠肝脏的抗氧化能力;组织病理学检测结果也表明,MRJPs可有效抑制CCl4诱导的急性肝损伤;MRJPs诱导CCl4诱导TNF-α基因水平增加(P<0.05),从而调控免疫系统。综上,蜂王浆主蛋白对CCl4引起的小鼠急性肝损伤具有保护作用。更多还原

【Abstract】 In order to explore the effects of MRJPs(Major royal jelly proteins),the effects of the main water-soluble component of RJ on carbon tetrachloride(CCl4)-induced liver injury were investigated.The CCl4-induced acute liver injury model was established.Mice were randomly divided into six groups including negative control,positive control(CCl4 treated control group),Drug control,MRJPs(480 mg/kg)plus CCl4 treated group,MRJPs(320 mg/kg)plus CCl4 treated group and MRJPs(160 mg/kg)plus CCl4 treated group,respectively.The mice were pre-treated orally with MRJPs(480,320 or 160 mg/kg)once daily for 28 days before CCl4(10 mL/kg of 0.2% CCl4 solution in olive oil)injection.The hepato-protective effect of MRJPs was evaluated by the biochemical parameters related to hepatic damages,such as ALT,AST,SOD,MDA and TNF-α.The results showed that:MRJPs inhibited serum ALT and AST activities compared with positive group;MRJPs remarkably increased the levels of SOD and decreased MDA in liver;Histopathological test results showed that MRJPs effectively inhibited the acute liver injury induced by CCl4.Further investigation demonstrated that MRJPs markedly increased the gene expression of TNF-αin liver.In conclusion,MRJPs protected mouse from CCl4-induced acute liver injury.更多还原