【摘要】 为开发黄麻SSR引物,为黄麻分子标记辅助育种等研究提供有利工具,在黄麻转录组测序的基础上,采用MicroSAtellite(MISA)软件进行SSRs搜索,并对部分SSR引物的多态性进行验证,结果表明:1)在1kb以上的13 718条unigene中,共检测到8 571个SSR位点,占评估序列数目的62.47%,平均每3.47kb有1个SSR;2)所开发的SSR标记中,单核苷酸重复序列最多,占42.15%,二核苷酸和三核苷酸序列也较丰富,分别占14.80%和16.74%;3)利用Primer3.0软件设计了与木质素合成酶基因及MYB转录因子相关的880对引物,随机选取29对引物,以8份不同类型黄麻种质进行多态性验证,获得8对具有稳定多态性的引物,多态性引物比率为27.58%。结果表明,基于黄麻转录组序列的SSR标记开发是可行的,开发的SSR标记可为黄麻属遗传多样性分析、遗传图谱构建及功能基因的挖掘等研究提供有利工具。更多还原

【Abstract】 In order to develop SSR markers and provide powerful tools for the molecular marker-assisted breeding of jute,the SSR loci from transcriptome sequences were searched by MicroSAtellite(MISA)software,and the polymorphism of some SSRs were identified.The result showed that:1)A total of 8 571 locus,which were longer than 1 kb and accounted for 62.47% of the screened Unigenes,were identified from 13 718 Unigenes,resulting in one SSR per 3.47 kb on average;2)Mononucleotide repeat was the most abundant repeat type,accounting for 42.15% of the total number of SSRs.The dinucleotide and trinucleotide repeat were less abundant,which ratio was 14.80% and 16.74%,respectively;3)A total of 880 primers related to lignin synthase gene and MYB transcription factor were designed by Primer 3.0,among which 29 were screened and 8 pairs of primers produced polymorphic bands.The ratio of polymorphic primers was 27.58%.In conclusion,it is feasible to develop SSR molecular markers based on transcriptome sequencing analysis of jute,which could be applied for analyzing genetic diversity,genetic linkage mapping and functional gene mining of jute.更多还原