【摘要】 为筛选出高效降胆固醇乳杆菌,挖掘其潜在益生功能,从民间自制泡菜样品中分离乳杆菌,采用邻苯二甲醛法筛选高效降胆固醇菌株,利用16S rDNA进行分子生物学鉴定,并通过耐酸、耐胆盐及抑菌性实验,测定菌株的益生潜能。结果表明:1)从分离出的11株乳杆菌中筛选出PL2、PL5、PL16和PL194等4株高效降解胆固醇菌株,其体外胆固醇降解率分别为48.22%、51.67%、48.43%和48.56%;2)结合菌落特征、部分生理生化试验及16S rDNA基因序列比对鉴定4株乳酸菌均为植物乳杆菌(Lactobacillus plantarum);3)该4株乳酸菌均可在pH2.0的环境下生存3h,菌株PL2和PL5可以在0.3%的胆盐浓度下存活3h;4)菌株PL5对致病性大肠杆菌、沙门氏菌、金黄色葡萄球菌和福氏志贺氏菌均有显著的抑制作用。总之,植物乳杆菌PL5是具有开发前景的高效降胆固醇益生菌。更多还原

【Abstract】 The aim of this study was to screen the Lactobacillus with efficient cholesterol-lowering ability and discover its beneficial potency.Eleven Lactobacillus strains were isolated from homemade pickle samples and screened for high cholesterol-lowering by phthalic aldehyde method and 16S rDNA molecular biology identification.The acid tolerance,bile tolerance and antibacterial tests were then conducted to analyze their probiotic properties.The results showed that:1)High cholesterol-lowering strains,PL2,PL5,PL16 and PL19,were obtained from the screen,and their cholesterol degradation rate were 48.22%,51.67%,48.43% and 48.56%,respectively.2)The four strains were identified as L.plantarum by morphological observation and molecular biology identification.3)All strains survived in pH 2.0 for three hours.Strains PL2 and PL5 survived under ambient of 0.3% bile salt concentration for three hours.4)It was found that strain PL5 displayed significant inhibitory effect on four common pathogens.In conclusion,strain PL5 was a potential probiotics with excellent cholesterol-lowering effect.更多还原