【摘要】 基于在pH 8.6的甘氨酸-氢氧化钠-乙腈体系中,硼氢化钠可以将孔雀石绿(MG)定量还原为隐性孔雀石绿(LMG)从而具有荧光,表面活性剂十二烷基磺酸钠(SDS)的加入能使LMG荧光强度增强并稳定,建立了测定水产品中MG和LMG的同步荧光分光光度法.用正交试验设计对测定的实验条件进行了优化,结果发现:0.70 m L的甘氨酸-氢氧化钠-乙腈体系(pH 8.6),0.30 m L 0.2 mol/L Na BH4,0.50 m L浓度为1×10-4mol/L的SDS,避光放置7 min,以同步波长差△λ=98 nm进行同步荧光扫描,MG浓度在1.0×10-7~1.0×10-6mol/L线性范围与其同步荧光强度成正比,相关系数r为0.998,检出限为2.4×10-8mol/L.应用于水产品中MG和LMG的测定,加标回收率为95.3%~101.3%,RSD为4.2%~6.7%.更多还原

【Abstract】 In the glycine-sodium-acetonitrile system with a pH value of 8.6,a strong reducing agent sodium borohydride could quantitatively transform malachite green(MG)to leucomalachite green(LMG)that could produce fluorescence. After the addition of surfactant sodium dodecyl sulphate(SDS),the fluorescence intensity of the system can be enhanced and stablized. Based on this principle,a new method for the simultaneous determination of MG and LMG in aquatic products by synchronous fluorescence was proposed. Experimental conditions were optimized by orthogonal test methods. The results showed that the best conditions were: 0.70 m L of glycine-sodium-acetonitrile system(pH 8.6),0.30 m L of 0.2 mol/L sodium borohydride,0.50 m L of 1×10-4 mol/L SDS,7 min of the storage in dark,and △λ =98 nm for synchronous scanning. In the range of 1.0 ×10-7~1.0×10-6 mol/L,the concentration of MG and the synchronous fluorescence intensity demonstrated a significant linearity with a correlation coefficient of0.998. The detection limit was 2.4×10-8 mol/L. The proposed method was used for determining MG and LMG in aquatic products. The sample recoveries ranged from 95.3% to 101.3%,and the relative standard deviations were4.2% ~ 6.7%.更多还原