【摘要】 目的:研究IQCG(IQ motif containing G)基因在人类睾丸和精子中的定位及表达,比较其在正常精子活力男性和弱精子症患者精子中的表达差异,探究其可能的作用方式及与生育力的关系。方法:采用实时定量PCR对IQCG基因在人类睾丸的表达进行研究,进而通过免疫组化和免疫荧光研究IQCG在人类睾丸和精子中的定位,另收集门诊正常精子活力和不同等级的弱精子症及AID成功助孕精子标本,从蛋白表达方面分析IQCG在精子中的表达差异。结果:免疫组化显示IQCG在人类睾丸组织中广泛表达,从精母细胞到精子细胞都有表达,且特异性地表达于精子尾部,在精原干细胞中表达很弱或不表达,精原细胞中表达强烈。精子免疫组化和免疫荧光显示IQCG在弱精子症精子中的表达较正常活力精子中的表达降低,有统计学差异(P=0.041)。Western印迹结果显示IQCG在正常精子和弱精子症及与正常生育男性精子中均有表达,且在正常精子活力和重度弱精子症组之间(P=0.032)及正常生育组和重度弱精子症组之间(P=0.027)的表达存在统计学意义差异。结论:IQCG基因和蛋白可能在精子运动方面发挥作用,其表达异常可能是导致人类精子运动能力下降和生育力下降的原因之一,研究其作用机制可能为人类弱精子症的病因和治疗提供新的方向。更多还原

【Abstract】 Objective:To investigate the expression and location of IQ motif-containing G(IQCG)in the human testis,compare its expression in normal-motility sperm with that in the sperm of asthenospermia patients,and explore its possible mechanisms and its correlation with fertility.Methods:The expression of the IQCG gene in the human testis was detected by RT-PCR and its location in the testis and sperm was determined by immunohistochemistry and immunofluorescence staining.Semen samples were collected from normal males,patients with asthenospermia,and fertile men that succeeded in artificial insemination with donor’s sperm(AID),followed by analysis of the IQCG protein expression in different groups of samples by Western blot.Results:Immunohistochemistry showed that IQCG was extensively expressed in the human testis,in the spermatocytes and spermatids,specifically in the sperm tail,weakly expressed or absent in the spermatogonial stem cells,and strongly expressed in the spermatogonial cells.The expression of IQCG was significantly lower in the asthenospermia patients than in the normal males(P=0.041).Western blot manifested that IQCG was expressed in the semen of all the three groups of subjects,with statistically significant differences between the normal men and severe asthenospermia patients(P=0.032)as well as between the fertile males and the severe asthenospermia group(P=0.027).Conclusion:IQCG may act on human sperm motility and its abnormal expression possibly reduces sperm motility and fertility.An insight into its action mechanisms may shed some new light on the etiology and treatment of asthenospermia.更多还原