【摘要】 目的:探讨早产儿呼吸窘迫综合征(respiratory distress syndrome,RDS)患儿外周血血清中miR-21的表达水平及其临床意义。方法:选取2015年6—12月入住南京医科大学第一附属医院新生儿监护病房的RDS患儿30例;对照组为非RDS早产儿共21例,使用逆转录PCR法检测外周血血清中miR-21的表达水平,并分析各组病例的临床特点。结果:RDS患儿血清中miR-21表达水平明显高于对照组,差异有统计学意义(P <0.05)。中重度RDS组miR-21表达水平有明显增加趋势,但目前尚未发现差异有统计学意义。RDS患儿中使用肺表面活性物质(pulmonary surfactant,PS)组miR-21表达水平明显增加(P <0.05)。结论:血清miR-21表达水平与RDS密切相关,提示miR-21在RDS发病机制中起一定作用。更多还原

【Abstract】 Objective:To determine the pathological significance of serum miR-21 expression level in premature respiratory distress syndrome(RDS)infants. Methods:Randomized blood samples were collected from RDS and non-RDS infants in the Neonatal Intensive Care Unit(NICU)of Jiangsu Province Hospital from June to December 2015. Total RNA was extracted from each blood sample and reverse transcribed into complementary DNA(c DNA). We used real-time reverse transcription polymerase chain reaction(RT-PCR)technique to determine the serum miR-21 expression levels in the RDS and the non-RDS groups(n=30 and n=21,respectively).Unpaired student t-test was used to compare the significance between groups. Results:The serum miR-21 expression level in the RDS group was significantly higher than that in the control group(P < 0.05). The miR-21 expression levels in the moderate to severe RDS groups appeared to be higher than that in the mild groups,however,statistic significance was not reached in our study. In addition,the RDS group that received pulmonary surfactant therapy had significant increase in miR-21 expression levels(P < 0.05). Conclusion:Serum miR-21 expression level is closely related to the development of infant RDS,suggesting that miR-21 is involved in the pathogenesis of infant RDS.更多还原