【摘要】 研究纤维素酶联合超声波协同提取四川攀枝花石榴皮总黄酮.探究了乙醇体积分数、料液比、酶活量、超声波功率、提取时间、提取温度等对总黄酮产率的影响.以正交试验设计优化出了最佳提取条件:酶活度为20U/mL、超声波功率为280 W、提取时间为20min、提取温度为60℃,此条件下总黄酮产率为8.805%,提取物对1,1-二苯基-2-三硝基苯肼自由基的清除能力强于维生素C和芦丁,其清除能力存在较好的量效关系.更多还原

【Abstract】 To study the synergistic extraction of flavonoids in Panzhihua pomegranate peel by ultrasonic-assisted cellulase method,the effects of ethanol volume fraction,solid-liquid ratio,enzymatic activity,ultrasonic power,extraction time and extraction temperature on the yield rate of total flavonoids are examined.By orthogonal experiment design,the optimal extraction conditions were acquired:when the enzyme activity is 20 U/mL,ultrasonic power is at 280 W,extraction time lasts 20 minutes,extraction temperature at 60℃,the yield rate of total flavonoids could reach 8.805%.The scavenging ability of extract for 1,1-diphenyl-2-picrylhydrazyl free radical was stronger than vitamin C and rutin and the scavenging ability was of good dose-effect relationship.The experiment method can serve as a good means for the better utilization of Panzhihua pomegranate peel resource.更多还原