【摘要】 为了研究邻苯二甲酸二乙基己酯(DEHP)对巨噬细胞的免疫毒性,采用不同浓度剂量DEHP处理小鼠巨噬细胞株RAW264.7细胞和腹腔巨噬细胞,通过检测细胞吞噬、细胞因子分泌及活性氧水平变化探讨DEHP暴露对免疫系统的毒性作用。结果表明:高浓度(20、40、80 mg·L-1)DEHP处理48 h后对RAW264.7细胞可造成急性损伤。低浓度(1、5、10 mg·L-1)DEHP连续染毒处理10代后,RAW264.7细胞吞噬红细胞百分率及吞噬指数显著抑制(P<0.05)。RAW264.7细胞分泌TNF-α、IL-12和IL-23等细胞因子能力明显降低,并表现出一定的剂量-效应关系。随着DEHP暴露浓度的增加,DEHP染毒造成RAW264.7细胞内活性氧产生,受损加剧。除此之外,DEHP暴露还能明显抑制小鼠腹腔巨噬细胞对红细胞的吞噬能力(P<0.05)。以上结果表明,DEHP暴露对巨噬细胞具有免疫抑制作用,损伤了巨噬细胞正常免疫功能发挥。更多还原

【Abstract】 To evaluate the potential immunotoxicity of di(2-ethylhexyl) phthalate(DEHP), RAW264.7 cells and peritoneal macrophages were treated with different concentrations of DEHP. Further, the phagocytosis efficiency and cytokine secretion and reactive oxygen species(ROS)production ability of RAW264.7 cells, and adherence ability and phagocytosis efficiency of peritoneal macrophages were determined.The results revealed that the phagocytosis efficiency of RAW264.7 cells treated with 1, 5 mg·L-1 and 10 mg·L-1 DEHP was significantly inhibited. The concentration of tumor necrosis factor(TNF)-α, interleukin(IL)-12, and IL-23 decreased significantly in the cell culture supernatant. Furthermore, there was a dose-effect relationship between DEHP exposure and concentration of TNF-α, IL-12, and IL-23. The production of ROS in DEHP-treated RAW264.7 cells was inhibited significantly with increase in the concentration of DEHP. Further,DEHP inhibited the adhesion ability and phagocytosis efficiency of peritoneal macrophages collected form DEHP-treated mice. These results indicate that DEHP treatment can inhibit the normal function of both RAW264.7 cells and peritoneal macrophages.更多还原