【摘要】 目的探讨饱和脂肪酸棕榈酸(PA)和不饱和脂肪酸油酸(OA)对INS-1β细胞增殖及凋亡的影响。方法INS-1β细胞体外培养,采用MTT法检测细胞增殖,Annexin-Ⅴ/FITC/PI双标记流式细胞术检测细胞凋亡。结果和对照组相比,两种浓度OA均能促进INS-1β细胞生长,其中0.25 mmol/L OA能显著促进细胞生长(<0.05);两种浓度PA对INS-1β细胞生长均有抑制作用,其中0.50 mmol/L PA有显著抑制作用(<0.01)。Z-VAD-fmk能对抗PA对INS-1β细胞生长的抑制作用,LY294002则能促进PA对INS-1β细胞生长的抑制作用,抑制OA对INS-1β细胞生长的促进作用。OA能对抗高糖环境所引起INS-1β细胞的凋亡,而PA能显著增加INS-1β细胞凋亡的比例。结论 PA抑制INS-1β细胞生长并促进其凋亡,而OA对INS-1β细胞则具有保护作用;PA引起的细胞凋亡有依赖Caspase介导的凋亡信号通路,而PI3K/Akt信号通路在胰岛细胞脂性凋亡中对维持应激细胞的生存具有重要意义。更多还原

【Abstract】 Objective To investigate the effect of saturated fatty acid palmitate(PA)and unsaturated fatty acid Oleate(OA) on proliferation and apoptosis of INS-1β-cell. Methods Rat INS-1β-cells were maintained in RPMI 1640,and then treated with different concentration of PA and OA. Cell proliferation was assessed by MTT colorimetric assay. Inverted microscope was used to observe the morphological changes of FFAs-treated cells. The flow cytometry was used to detect the FFAs-induced apoptosis of INS-1β cells by using double labeled Annexin-V/FITC/PI. Results Compared with the control group, both concentrations of OA promoted the growth of INS-1β cells, and 0.25 mmol/L OA could significantly promoted cell growth(P < 0.05).Both concentrations of PA inhibited the growth of INS-1β cells. Among them, 0.50 mmol/L PA had a significant inhibitory effect( P< 0.01). Z-VAD-fmk could inhibit the inhibitory effect of PA on the growth of INS-1β cells. LY294002 could promote the inhibitory effect of PA on the growth of INS-1β cells and inhibit the growth of INS-1β cells. OA could prevent apoptosis of INS-1β cells caused by high glucose environment, while PA c could significantly increase the proportion of INS-1β cell apoptosis. Conclusion INS-1β cells exposed to PA under serum-free conditions showed marked apoptosis and little proliferation, however OA can protect INS-1β cells. The apoptosis of INS-1β cells exposed to high concentration and chronic PA was Caspase-dependent. PI3 K-Akt signal pathway plays an important role in lipoapoptosis of cells by stimulating cell proliferation and inhibiting apoptosis.更多还原