目的探究c-FOXP3基因在肾透明细胞癌体外侵袭迁移中的效应,并分析其与临床预后之间的关系。方法查询The Cancer Genomic Atlas(TCGA)数据库探寻c-FOXP3与肾细胞癌临床预后之间的关系;利用Western blot技术测定常见的肾透明细胞癌细胞系中c-FOXP3的表达水平;采用慢病毒感染技术感染c-FOXP3高表达的肾癌细株786-O与ACHN建立FOXP3敲低的肾癌细胞模型;采用细胞划痕实验,分别观察c-FOXP3敲低的786-O、ACHN及对照组细胞迁移能力的差异;分别用Transwell小室检测c-FOXP3敲低的786-O和ACHN以及对照组细胞的体外迁移和侵袭能力;Western blot法检测癌细胞侵袭迁移相关指标,初步探索c-FOXP3对肾癌细胞侵袭、迁移效应的分子机制。结果 TCGA临床数据库显示c-FOXP3表达量与肾细胞癌患者的总生存率呈现较明显的负相关关系,c-FOXP3表达量越高的肾癌患者,总生存率越低。常见肾癌细胞系中,786-O和ACHN相对高表达c-FOXP3。细胞划痕实验和Transwell实验显示c-FOXP3敲低的786-O和ACHN体外侵袭、迁...
【英文摘要】
Objective To explore the role of c-FOXP3 in the invasiveness of clear cell renal cell carcinoma(ccRCC)in vitro and to analyze its relationship with the clinical outcomes.Methods The Cancer Genomic Atlas(TCGA)database was used to analyze the relationship between c-FOXP3 expression and clinical outcomes.The expression of c-FOXP3 in common ccRCC cell lines was detected with Western blot.The c-FOXP3 knockdown786-O,ACHN cell lines were established with lentiviruses transfection technique.The migration ability of...
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