【摘要】 目的 探讨隐丹参酮(CPT)对荷瘤小鼠顺铂(DDP)化疗后免疫功能低下的影响。方法 40只小鼠分为5组,每组8只,PC9细胞建移植瘤动物模型,分别为空白对照组组、模型组、CPT组(100mg/kg)、DDP组(2mg/kg)及联合用药(100mgCPT+2mg/kgDDP)组,隐丹参酮连续给药15d,顺铂2次/周给药(每周第1天、第4天),停药24h后,测脾脏指数、CCK8检测T细胞增殖率、流式细胞术检测T细胞亚群、ELISA法检测荷瘤小鼠血清中IFN-γ、IL-2的含量。结果 与空白对照组比较,模型组免疫功能受到明显抑制(P<0.01);与模型组比较,CPT可明显提高荷瘤小鼠脾脏指数、T细胞增殖率、CD3~+、CD8~+、NK细胞、IFN-γ、IL-2的表达(P<0.01),而DDP的作用相反(P<0.01或P<0.05);与顺铂组比较,CPT组、联合用药组脾脏指数、T细胞增殖率、CD3~+、NK细胞、IFN-γ、IL-2表达明显上升(P<0.01),而CD4~+、CD8~+、CD4~+/CD8~+变化不明显(P>0.05);与隐丹参酮组比较,联合用药组脾脏指数、T细胞亚群、IL-2等差异无统计学意义(P>0.05),T细胞增殖数量、IFN-γ差异有统计学意义(P<0.01或P<0.05)。结论 隐丹参酮可提高化疗后机体的免疫功能,其机制可能与隐丹参酮提高血清中IFN-γ、IL-2含量相关,对机体的免疫脏器也有一定的保护作用,具有一定的临床应用前景。更多还原

【Abstract】 Objective To study the immunodeficiency of cryptotanshinone( CPT) on post-chemotherapeutic tumor-bearing mice.Methods Lung adenocarcinoma PC9 cell transplanted tumor animal models were divided into five groups: control group, model group, CPT group(100 mg/kg),DDP group(2 mg/kg), and the combination group of CPT and DDP( 100 mg CPT+2 mg/kg DDP), each group had eight mice.Cryptotanshinone was administered continuously to 15 d, cisplatin 2 times/week(first days, fourth days per week). After the withdrawal of 24 h,the spleen index, the proliferation rate of T cells were measured by CCK8, the T cell subgroup was detected by flow cytometry, and the content of IFN-gamma and IL-2 in the serum of the tumor bearing mice was detected by ELISA. Results Compared with the blank control group, the immune function of the model group was significantly inhibited( P<0.01). Compared with the model group, the spleen index, T cell proliferation rate,CD3~+, CD8~+, NK cells, IFN-gamma and IL-2 expression were significantly increased by CPT in the tumor bearing mice( P<0.01), but the effect of DDP was opposite(P<0.01 or P<0.05). Compared with cisplatin group, the expression of spleen index, T cell proliferation rate, CD3~+, NK cell, IFN-gamma and IL-2 in CPT group and combined drug group increased significantly( P<0.01), while CD4~+, CD8~+ and CD4+/CD8~+ were not significant(P>0.05). Compared with the cryptotanshinone group, there was no significant difference in the spleen index, T cell subgroup and IL-2(P>0.05) in the combination group( P>0.05). The number of T cells and the difference of IFN-gamma were statistically significant(P<0.01 or P<0.05). Conclusion CPT improves immune function after chemotherapy, and its mechanism may be related to the increase of the content of IFN-gamma and IL-2 in serum by cryptotanshinone. It also has a certain protective effect on the immune organs of the body, and has a certain clinical application prospect.更多还原