【摘要】 目的 探讨炙马钱子对自身免疫性重症肌无力(EAMG)模型大鼠的免疫调节机制。方法 采用鼠源乙酰胆碱受体α亚基97-116肽段方法免疫Lewis大鼠建立EAMG模型,观察实验大鼠的临床表现,并将建模成功的EAMG大鼠随机分为模型组、强的松组及炙马钱子低、中、高剂量组,每组各6只。采用酶联免疫吸附试验(ELISA)检测各组大鼠血清白介素-4(IL-4)、白介素-6(IL-6)含量。结果 模型组大鼠血清IL-4和IL-6含量比空白对照组显著升高(P<0.01);炙马钱子治疗组较模型组有不同程度下降,中、高剂量组IL-6含量明显低于强的松组(P<0.01)。结论 炙马钱子能改善EAMG大鼠的免疫反应,在一定剂量范围内的炙马钱子在免疫调节方面表现优于强的松。更多还原

【Abstract】 Objective To investigate the immune regulation mechanism of nux vomica on experimental autoimmune myasthenia gravis(EAMG)rats. Methods Lewis rats were immunized with torpedo-derived 97-116 peptide of the AchR α-subunit in CFA. After the second immunization,the rats’ weights and symptoms were observed to judge the success of the EAMG model. Rats with EAMG were divided into model group, prednisone group, nux vomica groups(low-, medium-, and high-dosage), 6 rats in each group, and were given the corresponding treatment respectively.The levels of IL-4 and IL-6 were determined by using enzyme linked immunosorbent assay( ELISA).Results The levels of IL-4 and IL-6 were increased in model group( all P<0.01).The nux vomica treatment group compared with model group had different degree of decline.The level of IL-6 in medium-and high-dosage nux vomica groups was significantly lower than that in prednisone group( all P<0.01), but there was no significant difference between high-and medium-dosage nux vomica groups(P>0.05). Conclusion Nux vomica can reduce the levels of IL-4 and IL-6 in EAMG model, inhibit the activation. The effect of nux vomica is better than prednisone in improving immune adjustment in a certain dosage range.更多还原