【摘要】 提出了高效液相色谱-双波长法同时测定不同生长期的黄精中腺苷、人参皂苷Rb1、β-谷甾醇、香草酸和甘草素等5种活性化学成分含量的方法。样品5.000 0g经甲醇（70+30）溶液40mL超声提取30min,提取液以Diamonsil钻石C₁₈色谱柱为固定相,以0.1%（体积分数）甲酸溶液和0.1%（体积分数）甲酸-乙腈溶液的混合液为流动相进行梯度洗脱,双波长检测（λ₁=252nm,λ₂=280nm）。在优化的试验条件下,5种活性化学成分的质量浓度在0.5¹00mg·L^-1范围内与其峰面积呈线性关系,检出限（3S/N）为0.36⁰.47mg·kg^-1。按标准加入法进行回收试验,回收率在96.0%¹01%之间,其相对标准偏差（n=6）均小于3.0%。更多还原

【Abstract】 A method of simultaneous determination of 5 active chemical constituents（adenosine,ginsenoside Rb1,β-sitosterol,vanillic acid and liquiritigenin）in Polygonatum Sibiricum Red.at different growth stages was proposed by high performance liquid chromatography with dual wavelengths.The sample（5.000 0 g）was extracted ultrasonically for 30 min with 40 mL of methanol（70+30）solution.The extract was separated by HPLC with dual wavelengths（λ1=252 nm,λ2=280 nm）using Diamonsil C18 column as the stationary phase,and a mixture of 0.1%（φ）formic acid solution and 0.1%（φ）formic acid-acetonitrile solution in different volume ratios as the mobile phase for gradient elution.Under the optimized test conditions,linear relationships between values of peak area and mass concentration of 5 active chemical constituents were found in the range of 0.5^-100 mg·L^-1,with detection limits（3 S/N）in the range of 0.36-0.47 mg·kg^-1.Test for recovery was made by standard addition method,giving results in the range of 96.0%^-101% and RSDs（n=6）all less than 3.0%.更多还原