【摘要】 目的探讨微小RNA-378(miR-378)在乳腺癌组织中的表达及临床意义。方法收集2012年1月至2014年12月女性乳腺癌患者手术切除的105对癌组织和癌旁组织,采用实时荧光定量PCR(QPCR)检测上述组织中miR-378的表达并比较miR-378在癌组织和癌旁组织中的分布差异,进一步分析miR-378表达与乳腺癌临床病理特征的关系,根据随访资料分析不同miR-378水平患者的预后情况。结果 miR-378在乳腺癌组织中的表达水平为2. 745±0. 347,高于癌旁组织的1. 085±0. 126(P<0. 01)。miR-378水平与年龄、淋巴结转移、绝经、组织学分级、ER和PR表达均无关,而与肿瘤大小、TNM分期、HER-2表达、Ki-67表达和Nottingham预后指数有关(P<0. 05)。单因素分析发现,乳腺癌患者的总生存期(OS)与年龄、肿瘤大小、绝经、ER和PR表达均无关,而与淋巴结转移、TNM分期、组织学分级、HER-2表达、Ki-67表达、Nottingham预后指数和miR-378水平有关(P<0. 05); miR-378低表达者的中位OS为>60. 0个月,高于高表达者的41. 0个月,差异有统计学意义(P<0. 05)。结论 miR-378在乳腺癌中高表达且与肿瘤的发生、发展和预后有一定关系,在乳腺癌筛查及预后预测上有一定的潜在价值。更多还原

【Abstract】 Objective To investigate the expression of microRNA-378 (miR-378) in breast cancer tissues and its clinical significance. Methods From January 2012 to December 2014,105 pairs of cancerous and paracancerous tissues surgically removed from 105 patients with breast cancer were collected. Real-time fluorescence quantitative PCR (QPCR) was used to detect the expression of miR-378 in the above tissues and the distribution of miR-378 in cancerous tissues and paracancerous tissues were compared. The relationship between the expression of miR-378 and clinicopathological parameters of breast cancer was further analyzed. According to the follow-up data,the prognosis of different miR-378 levels was analyzed. Results Compared with paracancerous tissues,miR-378 expression was upregulated in breast cancer tissue (2. 745± 0. 347 vs. 1. 085 ± 0. 126,P < 0. 01). MiR-378 level was not related with age,lymph node metastasis,menopause,histological grade,ER and PR expression,but with tumor size,TNM stage,HER-2 expression,Ki-67 expression and Nottingham prognosis index (P<0. 05). Univariate analysis showed that overall survival (OS) was not associated with age,tumor size,menopause,ER and PR expression,but with lymph node metastasis,TNM stage,histological grade,HER-2 expression,Ki-67 expression,Nottingham prognostic index and miR-378 expression (P<0. 05). The median OS of patients with low expression of miR-378 was more than 60. 0 months,better than 41 months of patients with high expression of miR-378 (P<0. 05).Conclusion MiR-378 is highly expressed in breast cancer and related to tumor progress and prognosis in breast cancer. These findings indicate that miR-378 may be regarded as a potential biomarker for the detection of breast cancer.更多还原