【摘要】 目的探讨tRNA天冬氨酸甲基转移酶1(TRDMT1)蛋白在胃癌组织中表达情况,并分析其表达与临床病理特征和预后的关系。方法收集东南大学医学院附属江阴医院2012年1月至2013年12月手术切除的胃癌组织标本90例及对应癌旁组织35例,采用免疫组化SP法分别检测上述组织中TRDMT1蛋白的表达,同时分析其表达与临床病理特征和总生存期(OS)的关系。结果 TRDMT1在胃癌组织中的阳性表达率为55.6%(50/90),高于癌旁组织中的11.4%(4/35),差异有统计学意义(P<0.05)。TRDMT1表达与年龄和性别无关(P>0.05),与肿瘤大小、分化程度、侵犯深度、淋巴结转移和TNM分期有关(P<0.05)。TRDMT1阳性表达者的中位OS为33.0个月,显著低于TRDMT1阴性表达者的42.0个月(P<0.05)。Cox多因素分析显示,肿瘤大小、分化程度、侵犯深度、淋巴结转移、TNM分期和TRDMT1表达均为影响胃癌患者OS的独立因素。结论 TRDMT1在胃癌中表达升高,其可能在胃癌的发生、发展中起促进作用且对预测胃癌患者的预后具有一定意义。更多还原

【Abstract】 Objective To explore the expression of tRNA aspartic acid methyltransferase 1( TRDMT1) in gastric cancer tissues,and the relationship of its expression with clinical features as well as prognosis. Methods From January 2012 to December2013,90 cases of gastric cancer tissues and 35 cases of corresponding normal tissues were collected in Jiangyin Hospital Affiliated to Medical College of Southeast University. Immunohistochemical SP method was used to detect the expression of TRDMT1 in the above tissues. The relationship of TRDMT1 expression with clinical features as well as overall survival( OS) was also analyzed. Results The positive expression rate of TRDMT1 was 55. 6%( 50/90) in gastric cancer tissues,higher than 11. 4%( 4/35) of corresponding normal tissues( P < 0. 05). The expression of TRDMT1 in gastric cancer tissues was related with tumor size,differentiation,depth of invasion,lymph node metastasis and TNM staging( P < 0. 05),but not with age and gender( P > 0. 05). The median OS of patients with TRDMT1 positive expression was 33. 0 months,lower than 42. 0 months of patients with TRDMT1 negative expression( P < 0. 05). Multivariate Cox regression analysis showed that tumor size,differentiation,depth of invasion,lymph node metastasis,TNM staging and TRDMT1 expression were independent factors influencing OS in patients with gastric cancer( P < 0. 05). Conclusion The elevated expression of TRDMT1 may contribute to the occurrence and development of gastric cancer and predict the prognosis of cancer gastric.更多还原