【摘要】 目的:探讨双靶向融合肽中构成单元的连接方式对融合肽修饰的钛表面成骨细胞在力负载下功能的影响。方法:设计将GGG片段插入到融合肽TBP-RGD中,用多肽对钛片进行涂层修饰后,检测其表面生长的成骨细胞在机械负载刺激下基因表达。结果:基因c-fos的表达量在30 g或90 g的离心力刺激下时,TBP-RGD组最高(P<0.05);180 g力值时,TBP-GGG-RGD组最具有优势(P<0.05)。Cbfα1的表达量在三种力值负载下,最高峰值均出现在TBP-RGD组(P<0.05),TBP-GGG-RGD未能提高Cbfα1的表达。结论:双靶向融合肽TBP-RGD能促进其修饰钛片表面的成骨细胞在力学负载下的基因c-fos和Cbfα1表达;增加"linker"GGG结构并不能进一步加强融合肽功能单位的作用。更多还原

【Abstract】 Objective: To investigate the influence of the connection mode of the component units of the dual-targeting chimeric peptide on the function of osteoblast on the peptide coating titanium surface under mechanical loading. Methods: The GGG linker was designed to be inserted into the chimeric peptide TBP-RGD. Osteoblasts were growing on titanium disks coated with peptide and subjected to mechanical loading,and their gene expressions were detected. Results: The gene expression of c-fos was the highest in TBP-RGD group under the stimulation by 30 g or 90 g centrifugal force( P < 0. 05).When the force was 180 g,the TBP-GGG-RGD group had the best advantage( P < 0. 05). The highest peak value of Cbfα1 expression was found in the TBP-RGD group( P < 0. 05) while TBP-GGG-RGD did not increase the expression of Cbfα1.Conclusion: The dual-targeting chimeric peptide TBP-RGD could promote the gene expressions of c-fos and Cbfα1 of osteoblasts on coated titanium surface under mechanical loading. Inserting the structure of GGG linker didn’t further enhance the function of chimeric peptide TBP-GGG-RGD.更多还原