【摘要】 [目的]探讨间期荧光原位杂交技术检测慢性粒细胞白血病(CML)骨髓和外周血细胞的bcr-abl融合基因的临床价值,并和常规染色体分析方法相比较。[方法]应用FISH技术和常规染色体分析技术对CML患者及正常对照组骨髓和外周血细胞bcr-abl融合基因进行分析。[结果]FISH检测对照组骨髓和外周血细胞阳性率为0.8%～3.7%,其临床分界值为2.67%,CML慢性期患者骨髓和外周血细胞bcr-abl阳性率分别为(59.9±24.6)%和(66.4±21.9)%,加速期分别为(76.2±17.9)%和(84.8±13.2)%,急变期分别为(78.3±20.1)%和(79.5±19.9)%,两者和CML慢性期相比差异具有明显的统计学意义(P <0.05)。Pearson相关系数分析发现,外周血细胞和骨髓细胞融合基因bcr-abl阳性率呈现典型的线性关系,同时发现在临床症状缓解的患者中,经骨髓移植的CML患者较药物治疗患者有较高的分子生物学缓解率(P <0.05)。[结论]间期荧光原位杂交技术(FISH)较常规染色体分析技术(CC)可特异性的检测CML患者治疗前后微残留状态,具有较高的准确性、可靠性、灵敏性。更多还原

【Abstract】 [Objective]Comparing with the conventional chromosome analysis method, to explore the detection value on chronic myelogenous leukemia by interphase fluorescence in situ hybridization.[Methods ]FISH technology and the conventional chromosome analysis technique was applied to detect the expression of bcr-abl fusion gene of the bone marrow and peripheral blood cells of the CML patients.[Results]The positive rate of the control group bone marrow and peripheral blood cell is 0.8% ～3.7%, its clinical bondary value is 2.67%, the positively expression rate of bcr-abl fusion in peripheral and bone marrow cell was( 59.9± 24. 6) % and( 66. 4 ± 21.9) % of the patients with chronic phase, were 76. 2 ± 17. 9) % and( 84. 8 ± 13. 2) % in those with accelerating phase, and were( 78.3± 20.1) % and( 79.5± 19.9) % in those with blastic crisis, comparing with the CML chronic phase,the difference of the accelerating phase and the blastic crisis phase has obvious statistical significance( P < 0.05), the perason correlation coefficient analysis found that the expression of the fusion gene bcr-abl of the peripheral blood cells and bone marrow cell presents typical liner relationship. Moreover, the bone marrow transplant patients have higher remission rate than other groups( P < 0.05).[Conclusion]the interphase fluresence in situ hybrization( FISH) can detect bcr-abl fusion gene of CML patients after the treatment, and it has the high accuracy, reliability and sensitivity.更多还原