【摘要】 为了增大嗜酸氧化亚铁硫杆菌细胞密度以提高其对Fe²⁺的氧化速率,利用泰勒花环填料为载体,在喷淋塔中固定嗜酸氧化亚铁硫杆菌,考察固定化细胞对Fe²⁺的生物氧化性能。采用扫描电子显微镜对载体表面进行形貌表征,X射线衍射仪和傅里叶红外光谱仪对填料表面沉淀进行成分分析。结果表明:在改良的9 K培养基中,嗜酸氧化亚铁硫杆菌被成功的固定在泰勒花环填料上,并伴有少量黄铵铁矾沉淀,这些沉淀和菌体细胞一起构成了生物反应系统的生物膜。嗜酸氧化亚铁硫杆菌在喷淋塔中完成固定化后,溶液初始pH值和温度是影响氧化速率的主要原因,其优化结果分别为2.25和34℃。在此条件下,整个生物反应系统能长期保持稳定的氧化特性,Fe²⁺的氧化速率为4.27 g/L/h。更多还原

【Abstract】 For the purpose of improving oxidation rate of ferrous iron, Teller rosette was used as the immobilization carrier of Acidithiobacillus ferrooxidans in a spray tower reactor to improve its cells density. The performance of biological oxidation of ferrous iron by immobilized cells was studied. Characterizations were identified by SEM, XRD and FTIR. The results showed that Acidithiobacillus ferrooxidans was immobilized on carrier successfully, with some precipitation, ammoniojarosite. The film of bioreactor system consisted of precipitation and bacterial cells. After immobilization, the optimum operating conditions were p H 2.25 and 34°C. Biological oxidation ability of the system could stay a long-term stability level. The oxidation rate of ferrous iron was 4.27 g/L/h.更多还原