【摘要】 评价了桃种质资源的遗传多样性水平及遗传结构,为其保存提供理论依据。利用12对SSR引物对53份桃种质资源进行了遗传多样性分析,利用Neighbor-Joining聚类对其遗传结构进行了分析比较。结果表明:(1) 12对引物在53个样品中的平均等位基因数(Na)为7.917,Shannon多样性指数(I)为1.409,平均有效等位基因数(Ne)为3.399,平均观察杂合度(Ho)为0.657,期望杂合度(He)平均值为0.678。(2)基于Nei距离的Neighbor-Joining聚类分析表明:53个桃样品被分为3个组,同时能清楚地鉴别出同物异名的种质资源。由此可知,供试的桃种质资源表现出较高的遗传多样性水平;大部分遗传关系较近的品种以及部分形态学性状相近的品种聚在一类,说明聚类分析结果与系谱及生物学特征具有一定的关联性,同时聚类结果与品种的地理分布具有一定相关性。更多还原

【Abstract】 The genetic diversity and genetic structure of peach germplasm resources were assessed to provide theoretical basis for their conservation. Twelve pairs of SSR( Simple Sequence Repeats) primers were used to evaluate the genetic diversity of 53 peach accessions,and Neighbor-Joining clustering was used to analyze and compare their genetic structure. The results showed that:( 1) The mean number of polymorphic alleles( Na) of 12 pairs of primers in 53 peach accessions was 7.917,and the average number of effective alleles( Ne) per locus was 3.399; the Shannon diversity index( I) was 1.409; the average observed heterozygosity( Ho) was 0.657,and the average expected heterozygosity( He) was 0.678.( 2) The Neighbor-Joining clustering analysis based on Nei’s distance divided 53 peach samples into 3 groups,and it could clearly identify the synonyms of these germplasm resources. The tested peach germplasm resources exhibited a high level of genetic diversity,and most of the varieties with closer genetic relationship or some similar morphological characteristics were clustered into the same group,indicating that the results of clustering analysis were related to the genealogy and biological characteristics. At the same time,the clustering results were also correlated with the geographical distribution of the varieties.更多还原