【摘要】 从昆明市郊的杜鹃盆花种植基地采集炭疽病样品,经病原分离和纯化得到形态一致的6个菌株;通过形态学观察、分子生物学和致病性鉴定,将该杜鹃炭疽病的病原菌鉴定为博宁炭疽菌(Colletotrichum boninense)。研究了不同碳源、氮源、p H值、温度对该病原菌生长的影响,结果表明:该病原菌能有效利用多种碳源,最适合的为葡萄糖;有利于该病原菌生长的有机氮为蛋白胨,无机氮为硝酸钾;病原菌在15~35℃下均能生长,最适温度为25℃;适于病原菌生长的培养基p H值为6~8;适于产孢的培养基为MA培养基。更多还原

【Abstract】 The rhododendron anthracnose samples were collected from the planting base of rhododendron pot-flower in the suburb of Kunming city,and six strains with the same morphological characteristics were obtained through pathogen isolation and purification. These strains were identified as Colletotrichum boninense based on morphological observation,molecular biological characteristics and pathogenicity test. The effects of carbon source,nitrogen source,p H-value,and culture temperature on the growth of this pathogen were studied. The results showed that: this pathogen could effectively utilize many kinds of carbon sources,and the best carbon source was glucose; the optimal organic and inorganic nitrogen sources were peptone and potassium nitrate,respectively; the optimum culture temperature was 25 ℃; the best p H-value of medium for the growth of C. boninense was 6 ~ 8; the suitable medium for spore formation was MA medium.更多还原