【摘要】 以猴头菌子实体为原料,萃取物得率作为指标,采用CO₂超临界流体萃取技术,以萃取压力和CO₂流量等参数为考察因素,结合正交试验获得优化的萃取工艺:萃取压力为30MPa,温度为45℃,时间为1.5h,CO₂流量为20g/min,夹带剂（乙醇）与猴头菌子实体的物料比为5:1（mL:g）,在此条件下,萃取物得率为2.78%。与猴头菌子实体的醇提物相比,猴头菌子实体超临界萃取物具有更好的体外抗氧化能力和抗肿瘤活性,本研究结果为合理地开发和利用猴头菌子实体超临界萃取物产品提供科学的数据。更多还原

【Abstract】 Active substance from fruiting body of Hericium erinaceus was extracted using supercritical CO₂ fluid technology, and the effects of the extraction pressure and CO₂ flow rate in process of extraction and separation on extract yields were investigated by orthogonal experiment. The optimal conditions were extraction pressure of 30 MPa at 45°C, extraction duration of 1.5 h, and carbon dioxide flow rate of 20 g/min. The ratio of entrainer（ethanol） to H. erinaceus fruiting body weight was 5:1（mL:g）. Under these conditions, crude extract yield was 2.78%. The supercritical fluid CO₂ extracts of H. erinaceus fruiting bodies showed better antioxidant capability in vitro and anti-tumor activity as compared with ethanol extraction. These results provide fundamental data for exploiting functional products of the fungus.更多还原