【摘要】 采用皮层剥离法从东北大兴安岭的7种石蕊属地衣的15个样本的710个组织块分离获得90株地衣内生真菌菌株,平均定植率为12.68%(2.86%–32.76%),定植率和石蕊属的物种之间不存在明显对应关系。根据菌株的菌落、菌丝及分生孢子等特征从中选取30个作为代表,测定其ITS序列。结合分子序列和菌落特征,该90个菌株归属于19个分类单元,隶属于3门、5纲、8目、11科和15属。炭角菌科为优势科,占77.78%,紫棕炭团菌为优势种,占62.22%。每个石蕊种类及总体的内生真菌菌群的Shannon-Wiener指数和Simpson指数显示所获得的石蕊属地衣内生真菌菌群具有中等的多样性及较强的宿主专一性。更多还原

【Abstract】 Seven species of Cladonia were collected from the Greater Khingan Mountains, Northeast China. The endolichenic fungi associated with them were isolated by using the scraping cortex method and 90 strains were obtained from 710 pieces for 15 samples. The average colonization rate was 12.68%(2.86%–32.76%). There was no obvious corresponding relationship between the Cladonia species and its colonization rate. Based on morphological characteristics of colony, hyphae and conidia, 30 strains were selected as representatives and the ITS sequences of them were obtained and analyzed. According to the culturalcolony characteristics and ITS sequences, these strains were identified as 19 taxa belonging to 15 genera, eleven families, eight orders, and five classes in three phyla. Xylariaceae was the dominant family, accounting for 77.78% of all strains. Hypoxylon fuscum was the dominant species, accounting for 62.22%. The Shannon-Wiener and Simpson diversity index values of the fungal community for every Cladonia species and overall demonstrated that there was moderate endolichenic fungal species diversity in Cladonia with obvious host specificity.更多还原