【摘要】 目的 :采用小动物活体成像技术,观察过表达SASH1对荷胶质瘤裸鼠的抗肿瘤生长作用。方法 :将稳定表达GFP绿色荧光蛋白及荧光素酶报告基因(luciferase)的胶质瘤SHG-44细胞接种于裸鼠皮下,待成瘤后,将ADV4-SASH1慢病毒注射入裸鼠肿瘤组织中,对照组注射相同剂量的ADV4-NC空载体对照病毒;1周后,腹腔注射底物荧光素(luciferin),用小动物活体成像仪采集荧光值,分析肿瘤生长情况。结果:过表达SASH1的裸鼠中有70%(7/10)肿瘤减小;对照组中有71.4%(5/7)裸鼠肿瘤增加,且有30%(3/10)裸鼠死亡。过表达SASH1 1周后肿瘤大小减小至90%,对照组肿瘤增大至166%。结论:本实验表明活体成像技术可以实时观察荷瘤鼠异位接种的胶质瘤体的生长情况,初步结果表明在胶质瘤体中过表达SASH1基因可以抑制肿瘤的生长。更多还原

【Abstract】 Objective: To investigate th eeffect of SASH1 gene overexpression on glioma by using bioluminescent imaging of animal in vivo imaging system. Methods: Glioma bearing mice model was established by inoculatingsubcutaneously SHG-44 cells with stably expressing GFP-Luci gene. After neoplasm emergence, ADV4-SASH1 were injected into tumors, while control mice were treated with ADV4-NC virus. After 1 week, luciferin was injected and the bioluminescent values were collected by animals in vivo imaging system, and the growth of the tumor was analyzed. Results: 70% of the tumors growth of the nude mice with an injection of ADV4-SASH1 decreased, while in the nude mice with an injection of the control virus, 50% of the tumors growth increased, and 30% of the nude mice died. Conclusion: In this study, it is shown that in vivo imaging technique could trace the growth of inoculated glioma in the nude mice. The preliminary results showed that overexpression of SASH1 could inhibit the glioma growth in vivo.更多还原